Genome-Wide Analysis of ROS Antioxidant Genes in Resurrection Species Suggest an Involvement of Distinct ROS Detoxification Systems during Desiccation
Saurabh Gupta 1,*
, Yanni Dong 2, Paul P. Dijkwel 2, Bernd Mueller-Roeber 1,3,4 and Tsanko S. Gechev 4,5,*
, Yanni Dong 2, Paul P. Dijkwel 2, Bernd Mueller-Roeber 1,3,4 and Tsanko S. Gechev 4,5,*
1
Department Molecular Biology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Straße 24-25, Haus 20, 14476 Potsdam, Germany
2
Institute of Fundamental Sciences, Massey University, Tennent Drive, Palmerston North 4474, New Zealand
3
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany
4
Center of Plant Systems Biology and Biotechnology (CPSBB), Ruski Blvd. 139, Plovdiv 4000, Bulgaria
5
Department of Plant Physiology and Molecular Biology, University of Plovdiv, 24 Tsar Assen str., Plovdiv 4000, Bulgaria
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 3101; https://doi.org/10.3390/ijms20123101
Received: 31 May 2019 / Revised: 19 June 2019 / Accepted: 24 June 2019 / Published: 25 June 2019
(This article belongs to the Special Issue Oxidative Stress and Redox Regulation in Plants)
Abstract
Abiotic stress is one of the major threats to plant crop yield and productivity. When plants are exposed to stress, production of reactive oxygen species (ROS) increases, which could lead to extensive cellular damage and hence crop loss. During evolution, plants have acquired antioxidant defense systems which can not only detoxify ROS but also adjust ROS levels required for proper cell signaling. Ascorbate peroxidase (APX), glutathione peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) are crucial enzymes involved in ROS detoxification. In this study, 40 putative APX, 28 GPX, 16 CAT, and 41 SOD genes were identified from genomes of the resurrection species Boea hygrometrica, Selaginella lepidophylla, Xerophyta viscosa, and Oropetium thomaeum, and the mesophile Selaginella moellendorffii. Phylogenetic analyses classified the APX, GPX, and SOD proteins into five clades each, and CAT proteins into three clades. Using co-expression network analysis, various regulatory modules were discovered, mainly involving glutathione, that likely work together to maintain ROS homeostasis upon desiccation stress in resurrection species. These regulatory modules also support the existence of species-specific ROS detoxification systems. The results suggest molecular pathways that regulate ROS in resurrection species and the role of APX, GPX, CAT and SOD genes in resurrection species during stress. View Full-TextKeywords:
abiotic stress; desiccation; resurrection plants; ROS; ascorbate peroxidase; glutathione peroxidase; catalase; superoxide dismutase
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Gupta, S.; Dong, Y.; Dijkwel, P.P.; Mueller-Roeber, B.; Gechev, T.S. Genome-Wide Analysis of ROS Antioxidant Genes in Resurrection Species Suggest an Involvement of Distinct ROS Detoxification Systems during Desiccation. Int. J. Mol. Sci. 2019, 20, 3101.
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