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Article

Novel Insights into the Effect of Hyperforin and Photodynamic Therapy with Hypericin on Chosen Angiogenic Factors in Colorectal Micro-Tumors Created on Chorioallantoic Membrane

1
Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Šrobárova 2, 041 54 Košice, Slovakia
2
Cancer Research Institute BMC, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 3004; https://doi.org/10.3390/ijms20123004
Received: 10 May 2019 / Revised: 12 June 2019 / Accepted: 17 June 2019 / Published: 19 June 2019
(This article belongs to the Special Issue Insights into Photodynamic Therapy)
Photodynamic therapy with hypericin (HY-PDT) and hyperforin (HP) could be treatment modalities for colorectal cancer (CRC), but evidence of their effect on angiogenic factors in CRC is missing. Convenient experimental model utilization is essential for angiogenesis research. Therefore, not only 2D cell models, but also 3D cell models and micro-tumors were used and compared. The micro-tumor extent and interconnection with the chorioallantoic membrane (CAM) was determined by histological analyses. The presence of proliferating cells and HY penetration into the tumor mass were detected by fluorescence microscopy. The metabolic activity status was assessed by an colorimetric assay for assessing cell metabolic activity (MTT assay) and HY accumulation was determined by flow cytometry. Pro-angiogenic factor expression was determined by Western blot and quantitative real-time polymerase chain reaction (RT-qPCR). We confirmed the cytotoxic effect of HY-PDT and HP and showed that their effect is influenced by structural characteristics of the experimental model. We have pioneered a method for analyzing the effect of HP and cellular targeted HY-PDT on pro-angiogenic factor expression in CRC micro-tumors. Despite the inhibitory effect of HY-PDT and HP on CRC, the increased expression of some pro-angiogenic factors was observed. We also showed that CRC experimental micro-tumors created on quail CAM could be utilized for analyses of gene and protein expression. View Full-Text
Keywords: angiogenesis; hypericin; photodynamic therapy; hyperforin; colorectal cancer; pro-angiogenic factors; experimental models; micro-tumors; quail embryo angiogenesis; hypericin; photodynamic therapy; hyperforin; colorectal cancer; pro-angiogenic factors; experimental models; micro-tumors; quail embryo
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MDPI and ACS Style

Majerník, M.; Jendželovský, R.; Babinčák, M.; Košuth, J.; Ševc, J.; Tonelli Gombalová, Z.; Jendželovská, Z.; Buríková, M.; Fedoročko, P. Novel Insights into the Effect of Hyperforin and Photodynamic Therapy with Hypericin on Chosen Angiogenic Factors in Colorectal Micro-Tumors Created on Chorioallantoic Membrane. Int. J. Mol. Sci. 2019, 20, 3004. https://doi.org/10.3390/ijms20123004

AMA Style

Majerník M, Jendželovský R, Babinčák M, Košuth J, Ševc J, Tonelli Gombalová Z, Jendželovská Z, Buríková M, Fedoročko P. Novel Insights into the Effect of Hyperforin and Photodynamic Therapy with Hypericin on Chosen Angiogenic Factors in Colorectal Micro-Tumors Created on Chorioallantoic Membrane. International Journal of Molecular Sciences. 2019; 20(12):3004. https://doi.org/10.3390/ijms20123004

Chicago/Turabian Style

Majerník, Martin, Rastislav Jendželovský, Marián Babinčák, Ján Košuth, Juraj Ševc, Zuzana Tonelli Gombalová, Zuzana Jendželovská, Monika Buríková, and Peter Fedoročko. 2019. "Novel Insights into the Effect of Hyperforin and Photodynamic Therapy with Hypericin on Chosen Angiogenic Factors in Colorectal Micro-Tumors Created on Chorioallantoic Membrane" International Journal of Molecular Sciences 20, no. 12: 3004. https://doi.org/10.3390/ijms20123004

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