Genome Editing in Agriculture: Technical and Practical Considerations
AbstractThe advent of precise genome-editing tools has revolutionized the way we create new plant varieties. Three groups of tools are now available, classified according to their mechanism of action: Programmable sequence-specific nucleases, base-editing enzymes, and oligonucleotides. The corresponding techniques not only lead to different outcomes, but also have implications for the public acceptance and regulatory approval of genome-edited plants. Despite the high efficiency and precision of the tools, there are still major bottlenecks in the generation of new and improved varieties, including the efficient delivery of the genome-editing reagents, the selection of desired events, and the regeneration of intact plants. In this review, we evaluate current delivery and regeneration methods, discuss their suitability for important crop species, and consider the practical aspects of applying the different genome-editing techniques in agriculture. View Full-Text
Share & Cite This Article
Jansing, J.; Schiermeyer, A.; Schillberg, S.; Fischer, R.; Bortesi, L. Genome Editing in Agriculture: Technical and Practical Considerations. Int. J. Mol. Sci. 2019, 20, 2888.
Jansing J, Schiermeyer A, Schillberg S, Fischer R, Bortesi L. Genome Editing in Agriculture: Technical and Practical Considerations. International Journal of Molecular Sciences. 2019; 20(12):2888.Chicago/Turabian Style
Jansing, Julia; Schiermeyer, Andreas; Schillberg, Stefan; Fischer, Rainer; Bortesi, Luisa. 2019. "Genome Editing in Agriculture: Technical and Practical Considerations." Int. J. Mol. Sci. 20, no. 12: 2888.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.