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Genome Editing in Agriculture: Technical and Practical Considerations

Aachen-Maastricht Institute for Biobased Materials (AMIBM), Maastricht University, Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD Geleen, The Netherlands
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany
Indiana Biosciences Research Institute (IBRI), 1345 W. 16th St. Suite 300, Indianapolis, IN 46202, USA
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2888;
Received: 1 May 2019 / Revised: 29 May 2019 / Accepted: 6 June 2019 / Published: 13 June 2019
(This article belongs to the Special Issue Genome Editing in Plants)
The advent of precise genome-editing tools has revolutionized the way we create new plant varieties. Three groups of tools are now available, classified according to their mechanism of action: Programmable sequence-specific nucleases, base-editing enzymes, and oligonucleotides. The corresponding techniques not only lead to different outcomes, but also have implications for the public acceptance and regulatory approval of genome-edited plants. Despite the high efficiency and precision of the tools, there are still major bottlenecks in the generation of new and improved varieties, including the efficient delivery of the genome-editing reagents, the selection of desired events, and the regeneration of intact plants. In this review, we evaluate current delivery and regeneration methods, discuss their suitability for important crop species, and consider the practical aspects of applying the different genome-editing techniques in agriculture. View Full-Text
Keywords: base editors; oligonucleotide-directed mutagenesis; precision breeding; programmable nucleases; sequence-specific nucleases base editors; oligonucleotide-directed mutagenesis; precision breeding; programmable nucleases; sequence-specific nucleases
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Jansing, J.; Schiermeyer, A.; Schillberg, S.; Fischer, R.; Bortesi, L. Genome Editing in Agriculture: Technical and Practical Considerations. Int. J. Mol. Sci. 2019, 20, 2888.

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