Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain

Arribas-Blázquez, Marina; Olivos-Oré, Luis Alcides; Barahona, María Victoria; Sánchez de la Muela, Mercedes; Solar, Virginia; Jiménez, Esperanza; Gualix, Javier; McIntosh, J. Michael; Ferrer-Montiel, Antonio; Miras-Portugal, María Teresa; Artalejo, Antonio R.

doi:10.3390/ijms20010155

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Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain

Marina Arribas-Blázquez

^1,2,†,

Luis Alcides Olivos-Oré

^1,2,†,

María Victoria Barahona

^1,2,†,

Mercedes Sánchez de la Muela

³,

¹,

¹,

^2,4,

^5,6,

Antonio Ferrer-Montiel

⁷,

María Teresa Miras-Portugal

^2,4 and

Antonio R. Artalejo

^1,2,*

Department of Pharmacology and Toxicology, Veterinary Faculty, Universidad Complutense de Madrid, 28040 Madrid, Spain

Instituto Universitario de Investigación en Neuroquímica, Universidad Complutense de Madrid, 28040 Madrid, Spain

Department of Animal Medicine and Surgery, Veterinary Faculty, Universidad Complutense de Madrid, 28040 Madrid, Spain

⁴

Department of Biochemistry and Molecular Biology, Veterinary Faculty, Universidad Complutense de Madrid, 28040 Madrid, Spain

⁵

George E. Wahlen Veterans Affairs Medical Center, Salt Lake City, UT 84148, USA

⁶

Departments of Biology and Psychiatry, University of Utah, Salt Lake City, UT 84112, USA

⁷

Instituto de Biología Molecular y Celular (IBMC), Universitas Miguel Hernández, 03202 Elche, Spain

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2019, 20(1), 155; https://doi.org/10.3390/ijms20010155

Received: 13 December 2018 / Revised: 25 December 2018 / Accepted: 26 December 2018 / Published: 3 January 2019

(This article belongs to the Special Issue Membrane Channels in Human Diseases: The Spanish Ion Channel Initiative Consortium (SICI))

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Abstract

We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic constriction injury of the sciatic nerve. In slices of the adrenal gland from neuropathic animals, we have evidenced increased cholinergic innervation and spontaneous synaptic activity at the splanchnic nerve–chromaffin cell junction. Likewise, adrenomedullary chromaffin cells displayed enlarged acetylcholine-evoked currents with greater sensitivity to α-conotoxin RgIA, a selective blocker of α9 subunit-containing nicotinic acetylcholine receptors, as well as increased exocytosis triggered by voltage-activated Ca²⁺ entry. Altogether, these adaptations are expected to facilitate catecholamine output into the bloodstream. Last, but most intriguing, functional and immunohistochemical data indicate that P2X3 and P2X7 purinergic receptors and transient receptor potential vanilloid-1 (TRPV1) channels are overexpressed in chromaffin cells from neuropathic animals. These latter observations are reminiscent of molecular changes characteristic of peripheral sensitization of nociceptors following the lesion of a peripheral nerve, and suggest that similar phenomena can occur in other tissues, potentially contributing to behavioral manifestations of neuropathic pain. View Full-Text

Keywords: P2X3 receptors; P2X7 receptors; TRPV1 channels; α9 nicotinic acetylcholine receptors; neuropathic pain; chromaffin cells; adrenal medulla; stress

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MDPI and ACS Style

Arribas-Blázquez, M.; Olivos-Oré, L.A.; Barahona, M.V.; Sánchez de la Muela, M.; Solar, V.; Jiménez, E.; Gualix, J.; McIntosh, J.M.; Ferrer-Montiel, A.; Miras-Portugal, M.T.; Artalejo, A.R. Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain. Int. J. Mol. Sci. 2019, 20, 155. https://doi.org/10.3390/ijms20010155

AMA Style

Arribas-Blázquez M, Olivos-Oré LA, Barahona MV, Sánchez de la Muela M, Solar V, Jiménez E, Gualix J, McIntosh JM, Ferrer-Montiel A, Miras-Portugal MT, Artalejo AR. Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain. International Journal of Molecular Sciences. 2019; 20(1):155. https://doi.org/10.3390/ijms20010155

Chicago/Turabian Style

Arribas-Blázquez, Marina, Luis A. Olivos-Oré, María V. Barahona, Mercedes Sánchez de la Muela, Virginia Solar, Esperanza Jiménez, Javier Gualix, J. M. McIntosh, Antonio Ferrer-Montiel, María T. Miras-Portugal, and Antonio R. Artalejo. 2019. "Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain" International Journal of Molecular Sciences 20, no. 1: 155. https://doi.org/10.3390/ijms20010155

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Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain

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