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Methodological Approaches to Study Extracellular Vesicle miRNAs in Epstein–Barr Virus-Associated Cancers

Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USA
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Int. J. Mol. Sci. 2018, 19(9), 2810; https://doi.org/10.3390/ijms19092810
Received: 22 August 2018 / Revised: 14 September 2018 / Accepted: 14 September 2018 / Published: 18 September 2018
(This article belongs to the Special Issue The Role of MicroRNAs in Human Diseases)
Epstein Barr-virus (EBV) was the first virus identified to be associated with human cancer in 1964 and is found ubiquitously throughout the world’s population. It is now established that EBV contributes to the development and progression of multiple human cancers of both lymphoid and epithelial cell origins. EBV encoded miRNAs play an important role in tumor proliferation, angiogenesis, immune escape, tissue invasion, and metastasis. Recently, EBV miRNAs have been found to be released from infected cancer cells in extracellular vesicles (EVs) and regulate gene expression in neighboring uninfected cells present in the tumor microenvironment and possibly at distal sites. As EVs are abundant in many biological fluids, the viral and cellular miRNAs present within EBV-modified EVs may serve as noninvasion markers for cancer diagnosis and prognosis. In this review, we discuss recent advances in EV isolation and miRNA detection, and provide a complete workflow for EV purification from plasma and deep-sequencing for biomarker discovery. View Full-Text
Keywords: exosomes; oncosomes; microvesicles; microRNA; extracellular vesicle; herpesvirus exosomes; oncosomes; microvesicles; microRNA; extracellular vesicle; herpesvirus
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Sun, L.; Meckes, D.G., Jr. Methodological Approaches to Study Extracellular Vesicle miRNAs in Epstein–Barr Virus-Associated Cancers. Int. J. Mol. Sci. 2018, 19, 2810.

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