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Actin-Myosin Interaction: Structure, Function and Drug Discovery
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Int. J. Mol. Sci. 2018, 19(9), 2643;

Myosin Head Configurations in Resting and Contracting Murine Skeletal Muscle

BioCAT, Department of Biological Sciences, Illinois Institute of Technology, Chicago, IL 60616, USA
Author to whom correspondence should be addressed.
Received: 6 August 2018 / Revised: 29 August 2018 / Accepted: 3 September 2018 / Published: 6 September 2018
(This article belongs to the Special Issue The Actin-Myosin Interaction in Muscle)
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Transgenic mouse models have been important tools for studying the relationship of genotype to phenotype for human diseases, including those of skeletal muscle. We show that mouse skeletal muscle can produce high quality X-ray diffraction patterns establishing the mouse intact skeletal muscle X-ray preparation as a potentially powerful tool to test structural hypotheses in health and disease. A notable feature of the mouse model system is the presence of residual myosin layer line intensities in contracting mouse muscle patterns. This provides an additional tool, along with the I1,1/I1,0 intensity ratio, for estimating the proportions of active versus relaxed myosin heads under a given set of conditions that can be used to characterize a given physiological condition or mutant muscle type. We also show that analysis of the myosin layer line intensity distribution, including derivation of the myosin head radius, Rm, may be used to study the role of the super-relaxed state in myosin regulation. When the myosin inhibitor blebbistatin is used to inhibit force production, there is a shift towards a highly quasi-helically ordered configuration that is distinct from the normal resting state, indicating there are more than one helically ordered configuration for resting crossbridges. View Full-Text
Keywords: skeletal muscle; X-ray diffraction; actomyosin interaction; sarcomere structure; super-relaxed state skeletal muscle; X-ray diffraction; actomyosin interaction; sarcomere structure; super-relaxed state

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ma, W.; Gong, H.; Irving, T. Myosin Head Configurations in Resting and Contracting Murine Skeletal Muscle. Int. J. Mol. Sci. 2018, 19, 2643.

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