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Open AccessArticle

Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria

1
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Korea
2
Department of Food Science and Technology, Pukyong National University, Busan 48513, Korea
3
Department of Biomedical Engineering, Pukyong National University, Busan 48513, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(8), 2157; https://doi.org/10.3390/ijms19082157
Received: 29 June 2018 / Revised: 18 July 2018 / Accepted: 19 July 2018 / Published: 24 July 2018
(This article belongs to the Special Issue Chitins 2018)
The emergence of more virulent forms of human pathogenic bacteria with multi-drug resistance is a serious global issue and requires alternative control strategies. The current study focused on investigating the antibacterial and antibiofilm potential of ferulic acid-grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The result showed that CFA at 64 µg/mL concentration exhibits bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log colony forming units/mL reduction) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to altered membrane integrity and permeability. CFA dose dependently inhibited biofilm formation (52–89% range), metabolic activity (30.8–75.1% range) and eradicated mature biofilms, and reduced viability (71–82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-minimum inhibitory concentration (MIC) concentrations of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will offer insights into a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections. View Full-Text
Keywords: antibacterial agent; antibiofilm; ferulic acid grafted chitosan; human pathogenic bacteria antibacterial agent; antibiofilm; ferulic acid grafted chitosan; human pathogenic bacteria
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MDPI and ACS Style

Dasagrandhi, C.; Park, S.; Jung, W.-K.; Kim, Y.-M. Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria. Int. J. Mol. Sci. 2018, 19, 2157.

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