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Article

High-Content Screening of a Taiwanese Indigenous Plant Extract Library Identifies Syzygium simile leaf Extract as an Inhibitor of Fatty Acid Uptake

1
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
2
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
3
Research Center for Natural Products & Drug Development, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
4
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan
5
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(7), 2130; https://doi.org/10.3390/ijms19072130
Received: 21 June 2018 / Revised: 9 July 2018 / Accepted: 19 July 2018 / Published: 22 July 2018
(This article belongs to the Section Bioactives and Nutraceuticals)
Non-alcoholic fatty liver disease (NAFLD) has become the most common liver disease in the recent decades in both developed and developing countries, and is predicted to be the major etiology for liver transplantation in the next decade. Thus, pharmacological strategies to treat NAFLD are urgently needed. Natural products are considered an excellent source for drug discovery. By utilizing an image-based high-throughput screening with a library containing 3000 Taiwanese indigenous plant extracts, we discovered that the extract of Syzygium simile leaves (SSLE) has an anti-lipid droplet (LD) accumulation effect in hepatic cell lines. Analyses of the expression profile of genes involved in lipid metabolism revealed that SSLE suppressed the mRNA expression of CD36, fatty acid translocase. In agreement with this observation, we showed that SSLE inhibited CD36 protein expression and fatty acid uptake and has only limited effects on pre-formed LDs. Moreover, SSLE reduced LD accumulation and CD36 expression in enterocyte and macrophage cell lines. In conclusion, our findings suggest that SSLE could serve as a potential source for the discovery of novel therapeutic modalities for NAFLD and that the suppression of CD36 expression and fatty acid uptake could contribute to the lipid-lowering effect of SSLE. View Full-Text
Keywords: non-alcoholic fatty liver disease; lipid droplet; high-content screening; metabolic diseases; natural products non-alcoholic fatty liver disease; lipid droplet; high-content screening; metabolic diseases; natural products
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MDPI and ACS Style

Yen, C.-H.; Chang, H.-S.; Yang, T.-H.; Wang, S.-F.; Wu, H.-C.; Chen, Y.-C.; Lin, K.-J.; Wang, S. High-Content Screening of a Taiwanese Indigenous Plant Extract Library Identifies Syzygium simile leaf Extract as an Inhibitor of Fatty Acid Uptake. Int. J. Mol. Sci. 2018, 19, 2130. https://doi.org/10.3390/ijms19072130

AMA Style

Yen C-H, Chang H-S, Yang T-H, Wang S-F, Wu H-C, Chen Y-C, Lin K-J, Wang S. High-Content Screening of a Taiwanese Indigenous Plant Extract Library Identifies Syzygium simile leaf Extract as an Inhibitor of Fatty Acid Uptake. International Journal of Molecular Sciences. 2018; 19(7):2130. https://doi.org/10.3390/ijms19072130

Chicago/Turabian Style

Yen, Chia-Hung, Hsun-Shuo Chang, Tsai-Hsun Yang, Sheng-Fan Wang, Ho-Cheng Wu, Yu-Chang Chen, Kai-Jay Lin, and Sheena Wang. 2018. "High-Content Screening of a Taiwanese Indigenous Plant Extract Library Identifies Syzygium simile leaf Extract as an Inhibitor of Fatty Acid Uptake" International Journal of Molecular Sciences 19, no. 7: 2130. https://doi.org/10.3390/ijms19072130

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