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Open AccessArticle

The RNA Helicase DDX6 Associates with RIG-I to Augment Induction of Antiviral Signaling

Robert Koch-Institut, FG17—Division of Influenza Viruses and other Respiratory Viruses, 13353 Berlin, Germany
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(7), 1877;
Received: 21 December 2017 / Revised: 13 April 2018 / Accepted: 22 June 2018 / Published: 26 June 2018
Virus infections induce sensitive antiviral responses within the host cell. The RNA helicase retinoic acid-inducible gene I (RIG-I) is a key sensor of influenza virus RNA that induces the expression of antiviral type I interferons. Recent evidence suggests a complex pattern of RIG-I regulation involving multiple interactions and cellular sites. In an approach employing affinity purification and quantitative mass spectrometry, we identified proteins with increased binding to RIG-I in response to influenza B virus infection. Among them was the RIG-I related RNA helicase DEAD box helicase 6 (DDX6), a known component of cytoplasmic mRNA-ribonucleoprotein (mRNP) granules like P-bodies and stress granules (SGs). RIG-I and DDX6 both localized to the cytosol and were detected in virus-induced SGs. Coimmunoprecipitation assays detected a basal level of complexes harboring RIG-I and DDX6 that increased after infection. Functionally, DDX6 augmented RIG-I mediated induction of interferon (IFN)-β expression. Notably, DDX6 was found to bind viral RNA capable to stimulate RIG-I. These findings imply a novel function for DDX6 as an RNA co-sensor and signaling enhancer for RIG-I. View Full-Text
Keywords: DDX6; RIG-I; influenza; interferon DDX6; RIG-I; influenza; interferon
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MDPI and ACS Style

Núñez, R.D.; Budt, M.; Saenger, S.; Paki, K.; Arnold, U.; Sadewasser, A.; Wolff, T. The RNA Helicase DDX6 Associates with RIG-I to Augment Induction of Antiviral Signaling. Int. J. Mol. Sci. 2018, 19, 1877.

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