Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Microstructure Observations and Size Characterization
2.2. Percutaneous Penetration
2.3. Antioxidant Activity of UB-NLC against Oxidative Damage
2.4. Monitoring the Level of Reactive Oxygen Species in HaCaT Cells
2.5. Effect of UB-NLC on the Activity of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-PX)
2.6. Effect of UB-NLC on Lipid Peroxidation
3. Materials and Methods
3.1. Materials
3.2. Preparation of UB-NLC
3.3. Size Analysis
3.4. Freeze-Fracture Transmission Electron Microscopy (FF-TEM) Observations
3.5. High Performance Liquid Chromatography (HPLC) Analysis
3.6. Skin Deposition Study
3.7. Cell Culture
3.8. Cell Viability Assay
3.9. Time-Lapse Imaging Assays
3.10. Measurement of Reactive Oxygen Species (ROS)
3.11. Assay of Superoxide Dismutase (SOD) Activity
3.12. Glutathione Peroxidase (GSH-PX) Activity Assay
3.13. Determination of Malondialdehyde (MDA)
3.14. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Wang, J.; Wang, H.; Xia, Q. Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells. Int. J. Mol. Sci. 2018, 19, 1865. https://doi.org/10.3390/ijms19071865
Wang J, Wang H, Xia Q. Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells. International Journal of Molecular Sciences. 2018; 19(7):1865. https://doi.org/10.3390/ijms19071865
Chicago/Turabian StyleWang, Jianmin, Huiyun Wang, and Qiang Xia. 2018. "Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells" International Journal of Molecular Sciences 19, no. 7: 1865. https://doi.org/10.3390/ijms19071865