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Int. J. Mol. Sci. 2018, 19(7), 1863; https://doi.org/10.3390/ijms19071863

Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?

1
Department Chemistry Biomedical Sciences, Linnaeus University, SE-39182 Kalmar, Sweden
2
Department Kinesiology and Physical Education, McGill University, Montreal, QC H3A 2T5, Canada
*
Authors to whom correspondence should be addressed.
Received: 23 May 2018 / Revised: 19 June 2018 / Accepted: 20 June 2018 / Published: 25 June 2018
(This article belongs to the Special Issue The Actin-Myosin Interaction in Muscle)
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Abstract

In muscle, but not in single-molecule mechanics studies, actin, myosin and accessory proteins are incorporated into a highly ordered myofilament lattice. In view of this difference we compare results from single-molecule studies and muscle mechanics and analyze to what degree data from the two types of studies agree with each other. There is reasonable correspondence in estimates of the cross-bridge power-stroke distance (7–13 nm), cross-bridge stiffness (~2 pN/nm) and average isometric force per cross-bridge (6–9 pN). Furthermore, models defined on the basis of single-molecule mechanics and solution biochemistry give good fits to experimental data from muscle. This suggests that the ordered myofilament lattice, accessory proteins and emergent effects of the sarcomere organization have only minor modulatory roles. However, such factors may be of greater importance under e.g., disease conditions. We also identify areas where single-molecule and muscle data are conflicting: (1) whether force generation is an Eyring or Kramers process with just one major power-stroke or several sub-strokes; (2) whether the myofilaments and the cross-bridges have Hookean or non-linear elasticity; (3) if individual myosin heads slip between actin sites under certain conditions, e.g., in lengthening; or (4) if the two heads of myosin cooperate. View Full-Text
Keywords: optical tweezers; optical traps; muscle fiber; myofibril; myosin; actin; cross-bridge; mechanochemical model optical tweezers; optical traps; muscle fiber; myofibril; myosin; actin; cross-bridge; mechanochemical model
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Månsson, A.; Ušaj, M.; Moretto, L.; Rassier, D.E. Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle? Int. J. Mol. Sci. 2018, 19, 1863.

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