Effects of Cyclic Mechanical Stretch on the Proliferation of L6 Myoblasts and Its Mechanisms: PI3K/Akt and MAPK Signal Pathways Regulated by IGF-1 Receptor
Abstract
1. Introduction
2. Results
2.1. Effects of Cyclic Mechanical Stretch on the Proliferation of L6 Myoblasts
2.2. Effects of Cyclic Mechanical Stretch on the Expressions and Activities of PI3K/Akt and MAPKs (p38 and ERK1/2) in L6 Myoblasts
2.3. Pro-Proliferative Effect of 15% Mechanical Stretch on L6 Myoblasts Was Reversed by PI3K/Akt and ERK1/2 Inhibitors Rather Than p38 Inhibitor
2.4. Effects of Cyclic Mechanical Stretch on IGF-1 Secretion and IGF-1R Protein Level of L6 Myoblasts
2.5. IGF-1 Recombinant Peptide Alleviated 20% Stretch-Induced Proliferation Inhibition of L6 Myoblasts, Accompanied with the Increase of IGF-1R Protein Level as Well as Enhancements of PI3K/Akt and MAPKs (p38 and ERK1/2) Activities
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cyclic Mechanical Stretch
4.3. Detection of Cell Proliferation
4.4. Determination of IGF-1 Concentration in Cell Culture Supernatant
4.5. Treatments of L6 Myoblasts with IGF-1 Recombinant Peptide, and Specific Inhibitors of PI3K, p38, and ERK1/2
4.6. Western Blot
4.7. Statistical Analyses
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Fu, S.; Yin, L.; Lin, X.; Lu, J.; Wang, X. Effects of Cyclic Mechanical Stretch on the Proliferation of L6 Myoblasts and Its Mechanisms: PI3K/Akt and MAPK Signal Pathways Regulated by IGF-1 Receptor. Int. J. Mol. Sci. 2018, 19, 1649. https://doi.org/10.3390/ijms19061649
Fu S, Yin L, Lin X, Lu J, Wang X. Effects of Cyclic Mechanical Stretch on the Proliferation of L6 Myoblasts and Its Mechanisms: PI3K/Akt and MAPK Signal Pathways Regulated by IGF-1 Receptor. International Journal of Molecular Sciences. 2018; 19(6):1649. https://doi.org/10.3390/ijms19061649
Chicago/Turabian StyleFu, Shaoting, Lijun Yin, Xiaojing Lin, Jianqiang Lu, and Xiaohui Wang. 2018. "Effects of Cyclic Mechanical Stretch on the Proliferation of L6 Myoblasts and Its Mechanisms: PI3K/Akt and MAPK Signal Pathways Regulated by IGF-1 Receptor" International Journal of Molecular Sciences 19, no. 6: 1649. https://doi.org/10.3390/ijms19061649
APA StyleFu, S., Yin, L., Lin, X., Lu, J., & Wang, X. (2018). Effects of Cyclic Mechanical Stretch on the Proliferation of L6 Myoblasts and Its Mechanisms: PI3K/Akt and MAPK Signal Pathways Regulated by IGF-1 Receptor. International Journal of Molecular Sciences, 19(6), 1649. https://doi.org/10.3390/ijms19061649