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Open AccessArticle

Characterization of an Integrated Active Glu-1Ay Allele in Common Wheat from Wild Emmer and Its Potential Role in Flour Improvement

1
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
2
Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(4), 923; https://doi.org/10.3390/ijms19040923
Received: 25 February 2018 / Revised: 14 March 2018 / Accepted: 14 March 2018 / Published: 21 March 2018
(This article belongs to the Section Molecular Plant Sciences)
Glu-1Ay, one of six genes encoding a high molecular weight glutenin subunit (HMW-GS), is frequently silenced in hexaploid common wheat. Here, an active allele of Glu-1Ay was integrated from wild emmer wheat (Triticum turgidum ssp. dicoccoides) accession D97 into the common wheat (Triticum aestivum) cultivar Chuannong 16 via the repeated self-fertilization of the pentaploid interspecific hybrid, culminating in the selection of a line TaAy7-40 shown to express the wild emmer Glu-1Ay allele. The open reading frame of this allele was a 1830 bp long sequence, demonstrated by its heterologous expression in Escherichia coli to encode a 608-residue polypeptide. Its nucleotide sequence was 99.2% identical to that of the sequence within the wild emmer parent. The TaAy7-40 introgression line containing the active Glu-1Ay allele showed higher protein content, higher sodium dodecyl sulfate (SDS) sedimentation value, higher content of wet gluten in the flour, higher grain weight, and bigger grain size than Chuannong 16. The end-use quality parameters of the TaAy7-40 were superior to those of the medium gluten common wheat cultivars Mianmai 37 and Neimai 9. Thus, the active Glu-1Ay allele might be of potential value in breeding programs designed to improve wheat flour quality. View Full-Text
Keywords: Triticum turgidum ssp. dicoccoides; Triticum aestivum; introgression line; high molecular weight glutenin subunit; Glu-1Ay; distant hybridization; flour quality Triticum turgidum ssp. dicoccoides; Triticum aestivum; introgression line; high molecular weight glutenin subunit; Glu-1Ay; distant hybridization; flour quality
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Wang, Z.; Huang, L.; Wu, B.; Hu, J.; Jiang, Z.; Qi, P.; Zheng, Y.; Liu, D. Characterization of an Integrated Active Glu-1Ay Allele in Common Wheat from Wild Emmer and Its Potential Role in Flour Improvement. Int. J. Mol. Sci. 2018, 19, 923.

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