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Int. J. Mol. Sci. 2018, 19(4), 1206; https://doi.org/10.3390/ijms19041206

MicroRNAs Targeting Caspase-3 and -7 in PANC-1 Cells

1
Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University, Chuncheon 24252, Korea
2
Department of Physiology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA
3
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA
*
Authors to whom correspondence should be addressed.
Received: 6 March 2018 / Revised: 4 April 2018 / Accepted: 10 April 2018 / Published: 16 April 2018
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Abstract

MicroRNAs (miRNAs), a critical part of the RNA silencing machinery, are known to play important regulatory roles in cancer. However, the consequence of miRNA deregulation in cancer is unknown for many miRNAs. Here, we define that miRNAs, miR-17-5p, miR-132-3p/-212-3p, and miR-337-3p are significantly up-regulated in the pancreatic ductal adenocarcinomas (PDAC) compared to the normal and benign tissues. Furthermore, by using PANC-1 cells, we demonstrate that overexpressed miR-337-3p and miR-17-5p/miR-132-3p/-212-3p can regulate executioner caspases-3 and -7, respectively. In addition, over-expression of miRNAs, especially miR-337-3p, attenuates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity in PANC-1 cells. Our findings unveil an important biological function for miRNAs up-regulated in PDAC in coordinately regulating caspases, potentially contributing to the malignant progression of PDAC. View Full-Text
Keywords: microRNA; caspase-3; caspase-7; TRAIL; pancreatic adenocarcinomas microRNA; caspase-3; caspase-7; TRAIL; pancreatic adenocarcinomas
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Park, J.K.; Doseff, A.I.; Schmittgen, T.D. MicroRNAs Targeting Caspase-3 and -7 in PANC-1 Cells. Int. J. Mol. Sci. 2018, 19, 1206.

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