Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies
Abstract
:1. Introduction
2. Results
2.1. Construction of Vectors
2.2. Expression in a Eukaryotic System
2.3. Interaction Analysis of cGKIα and RGS2
2.4. Comparison of Expression Level of cGKIα with Tissue Expression and Analysis of Activity
2.5. Comparison of Different Stimuli
2.6. Analysis of Specificity
2.7. Analysis of Selectivity
2.8. Dimerization of cGKIα upon Oxidant Stimulation
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Construction of Vectors
4.3. Cell Culture and Transfection
4.4. Western Blotting
4.5. Luminescence Assay
4.6. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Schramm, A.; Mueller-Thuemen, P.; Littmann, T.; Harloff, M.; Ozawa, T.; Schlossmann, J. Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies. Int. J. Mol. Sci. 2018, 19, 1180. https://doi.org/10.3390/ijms19041180
Schramm A, Mueller-Thuemen P, Littmann T, Harloff M, Ozawa T, Schlossmann J. Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies. International Journal of Molecular Sciences. 2018; 19(4):1180. https://doi.org/10.3390/ijms19041180
Chicago/Turabian StyleSchramm, Andrea, Philip Mueller-Thuemen, Timo Littmann, Manuela Harloff, Takeaki Ozawa, and Jens Schlossmann. 2018. "Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies" International Journal of Molecular Sciences 19, no. 4: 1180. https://doi.org/10.3390/ijms19041180