Int. J. Mol. Sci. 2018, 19(4), 1150; https://doi.org/10.3390/ijms19041150
Localization Microscopy of Actin Cytoskeleton in Human Platelets
Sandra Mayr 1,*
, Fabian Hauser 1, Anja Peterbauer 2, Andreas Tauscher 1, Christoph Naderer 1, Markus Axmann 3, Birgit Plochberger 1 and Jaroslaw Jacak 1
, Fabian Hauser 1, Anja Peterbauer 2, Andreas Tauscher 1, Christoph Naderer 1, Markus Axmann 3, Birgit Plochberger 1 and Jaroslaw Jacak 11
School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria, Garnisonstr. 21, 4020 Linz, Austria
2
Red Cross Blood Transfusion Service for Upper Austria, Krankenhausstr. 7, 4017 Linz, Austria
3
Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Received: 23 February 2018 / Revised: 3 April 2018 / Accepted: 5 April 2018 / Published: 11 April 2018
(This article belongs to the Special Issue Single Cell Technology)
Abstract
Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm. View Full-TextKeywords:
actin cytoskeleton; Alexa Fluor 488; drift correction; dSTORM; localization microscopy; rhodamine; photo-switching; platelet shape change
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Mayr, S.; Hauser, F.; Peterbauer, A.; Tauscher, A.; Naderer, C.; Axmann, M.; Plochberger, B.; Jacak, J. Localization Microscopy of Actin Cytoskeleton in Human Platelets. Int. J. Mol. Sci. 2018, 19, 1150.
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