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Volume 19
Issue 4
10.3390/ijms19041150
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Open AccessCommunication

Localization Microscopy of Actin Cytoskeleton in Human Platelets

by
Sandra Mayr
1,*,
Fabian Hauser
1,
Anja Peterbauer
2,
Andreas Tauscher
1,
Christoph Naderer
1,
Markus Axmann
3,
Birgit Plochberger
1 and
Jaroslaw Jacak
1
1
School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria, Garnisonstr. 21, 4020 Linz, Austria
2
Red Cross Blood Transfusion Service for Upper Austria, Krankenhausstr. 7, 4017 Linz, Austria
3
Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(4), 1150; https://doi.org/10.3390/ijms19041150
Received: 23 February 2018 / Revised: 3 April 2018 / Accepted: 5 April 2018 / Published: 11 April 2018
(This article belongs to the Special Issue Single Cell Technology)
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Abstract

Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm. View Full-Text
Keywords: actin cytoskeleton; Alexa Fluor 488; drift correction; dSTORM; localization microscopy; rhodamine; photo-switching; platelet shape change actin cytoskeleton; Alexa Fluor 488; drift correction; dSTORM; localization microscopy; rhodamine; photo-switching; platelet shape change
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MDPI and ACS Style

Mayr, S.; Hauser, F.; Peterbauer, A.; Tauscher, A.; Naderer, C.; Axmann, M.; Plochberger, B.; Jacak, J. Localization Microscopy of Actin Cytoskeleton in Human Platelets. Int. J. Mol. Sci. 2018, 19, 1150. https://doi.org/10.3390/ijms19041150

AMA Style

Mayr S, Hauser F, Peterbauer A, Tauscher A, Naderer C, Axmann M, Plochberger B, Jacak J. Localization Microscopy of Actin Cytoskeleton in Human Platelets. International Journal of Molecular Sciences. 2018; 19(4):1150. https://doi.org/10.3390/ijms19041150

Chicago/Turabian Style

Mayr, Sandra; Hauser, Fabian; Peterbauer, Anja; Tauscher, Andreas; Naderer, Christoph; Axmann, Markus; Plochberger, Birgit; Jacak, Jaroslaw. 2018. "Localization Microscopy of Actin Cytoskeleton in Human Platelets" Int. J. Mol. Sci. 19, no. 4: 1150. https://doi.org/10.3390/ijms19041150

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