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Article

Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption

1
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
2
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 699; https://doi.org/10.3390/ijms19030699
Received: 13 February 2018 / Revised: 26 February 2018 / Accepted: 27 February 2018 / Published: 1 March 2018
(This article belongs to the Special Issue Nanotoxicology and Nanosafety)
The excellent biocompatibility and biological effects of fullerenol and its derivatives make their biomedical application promising. The potential effects of fullerenol in mammals have been extensively studied, but little is known about its effects on female reproduction. Using canonical oocyte-granulosa cell complexes (OGCs) in vitro maturation culture model, we investigated the effect of fullerenol on the first oocyte meiotic resumption. In the surrounding granulosa cells, fullerenol nanoparticles occluded the extracellular domain of the epidermal growth factor receptor (EGFR) to reduce EGFR-ligand binding and subsequent extracellular signal-regulated kinase 1 and 2 (ERK1/2) activation, which involved the regulation of connexin 43 (CX43) expression and internalization. Downregulation of CX43 expression and the retraction of transzonal projections (TZPs) interrupted the gap junction channel and TZPs based mass transportation. This effect decreased cyclic adenosine monophosphate (cAMP) levels in the oocyte and thereby accelerated rat oocyte meiosis resumption. Moreover, perinuclear distribution of CX43 and EGFR was observed in granulosa cells, which could further exacerbate the effects. Fullerenol nanoparticles interfered with the strict process of oocyte meiosis resumption, which likely reduced the oocyte quality. View Full-Text
Keywords: fullerenol; oocyte meiosis resumption; gap junction; granulosa cells; EGFR/ERK1/2 fullerenol; oocyte meiosis resumption; gap junction; granulosa cells; EGFR/ERK1/2
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MDPI and ACS Style

Lei, R.; Bai, X.; Chang, Y.; Li, J.; Qin, Y.; Chen, K.; Gu, W.; Xia, S.; Zhang, J.; Wang, Z.; Xing, G. Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption. Int. J. Mol. Sci. 2018, 19, 699. https://doi.org/10.3390/ijms19030699

AMA Style

Lei R, Bai X, Chang Y, Li J, Qin Y, Chen K, Gu W, Xia S, Zhang J, Wang Z, Xing G. Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption. International Journal of Molecular Sciences. 2018; 19(3):699. https://doi.org/10.3390/ijms19030699

Chicago/Turabian Style

Lei, Runhong, Xue Bai, Yanan Chang, Juan Li, Yanxia Qin, Kui Chen, Weihong Gu, Shibo Xia, Jiaxin Zhang, Zhenbo Wang, and Gengmei Xing. 2018. "Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption" International Journal of Molecular Sciences 19, no. 3: 699. https://doi.org/10.3390/ijms19030699

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