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Int. J. Mol. Sci. 2018, 19(3), 676; https://doi.org/10.3390/ijms19030676

The New Structure of Core Oligosaccharide Presented by Proteus penneri 40A and 41 Lipopolysaccharides

1
Laboratory of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL-90-237 Lodz, Poland
2
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland
3
Department of Biotechnology and Molecular Biology, University of Opole, PL-45-035 Opole, Poland
4
Department of Biology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz, Poland
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 29 January 2018 / Revised: 16 February 2018 / Accepted: 22 February 2018 / Published: 28 February 2018
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
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Abstract

The new type of core oligosaccharide in Proteus penneri 40A and 41 lipopolysaccharides has been investigated by 1H and 13C NMR spectroscopy, electrospray ionization mass spectrometry and chemical methods. Core oligosaccharides of both strains were chosen for structural analysis based on the reactivity of LPSs with serum against P. penneri 40A core oligosaccharide–diphtheria toxoid conjugate. Structural analyses revealed that P. penneri 40A and 41 LPSs possess an identical core oligosaccharide. View Full-Text
Keywords: anti-conjugate serum; core oligosaccharide; lipopolysaccharide; NMR spectroscopy; ESI MS; Proteus penneri anti-conjugate serum; core oligosaccharide; lipopolysaccharide; NMR spectroscopy; ESI MS; Proteus penneri
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Palusiak, A.; Maciejewska, A.; Lugowski, C.; Rozalski, A.; Kaszowska, M. The New Structure of Core Oligosaccharide Presented by Proteus penneri 40A and 41 Lipopolysaccharides. Int. J. Mol. Sci. 2018, 19, 676.

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