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Expression Profiling of Regulatory and Biosynthetic Genes in Contrastingly Anthocyanin Rich Strawberry (Fragaria × ananassa) Cultivars Reveals Key Genetic Determinants of Fruit Color

1
Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
2
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
3
University-Industry Cooperation Foundation, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
4
Department of Crop Science, Chungbuk National University, Chengju 28644, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 656; https://doi.org/10.3390/ijms19030656
Received: 27 January 2018 / Revised: 16 February 2018 / Accepted: 22 February 2018 / Published: 26 February 2018
(This article belongs to the Section Molecular Plant Sciences)
Anthocyanins are the resultant end-point metabolites of phenylapropanoid/flavonoid (F/P) pathway which is regulated at transcriptional level via a series of structural genes. Identifying the key genes and their potential interactions can provide us with the clue for novel points of intervention for improvement of the trait in strawberry. We profiled the expressions of putative regulatory and biosynthetic genes of cultivated strawberry in three developmental and characteristically colored stages of fruits of contrastingly anthocyanin rich cultivars: Tokun, Maehyang and Soelhyang. Besides FaMYB10, a well-characterized positive regulator, FaMYB5, FabHLH3 and FabHLH3-delta might also act as potential positive regulators, while FaMYB11, FaMYB9, FabHLH33 and FaWD44-1 as potential negative regulators of anthocyanin biosynthesis in these high-anthocyanin cultivars. Among the early BGs, Fa4CL7, FaF3H, FaCHI1, FaCHI3, and FaCHS, and among the late BGs, FaDFR4-3, FaLDOX, and FaUFGT2 showed significantly higher expression in ripe fruits of high anthocyanin cultivars Maehyang and Soelhyang. Multivariate analysis revealed the association of these genes with total anthocyanins. Increasingly higher expressions of the key genes along the pathway indicates the progressive intensification of pathway flux leading to final higher accumulation of anthocyanins. Identification of these key genetic determinants of anthocyanin regulation and biosynthesis in Korean cultivars will be helpful in designing crop improvement programs. View Full-Text
Keywords: Fragaria × ananassa; anthocyanin; flavonoid; transcriptional regulation; biosynthetic genes Fragaria × ananassa; anthocyanin; flavonoid; transcriptional regulation; biosynthetic genes
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Hossain, M.R.; Kim, H.-T.; Shanmugam, A.; Nath, U.K.; Goswami, G.; Song, J.-Y.; Park, J.-I.; Nou, I.-S. Expression Profiling of Regulatory and Biosynthetic Genes in Contrastingly Anthocyanin Rich Strawberry (Fragaria × ananassa) Cultivars Reveals Key Genetic Determinants of Fruit Color. Int. J. Mol. Sci. 2018, 19, 656.

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