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Int. J. Mol. Sci. 2018, 19(2), 628; https://doi.org/10.3390/ijms19020628

Epigallocatechin Gallate Reduces Ischemia/Reperfusion Injury in Isolated Perfused Rabbit Hearts

1
Heart Centre Clinic for Paediatric Cardiology, University of Leipzig, 04289 Leipzig, Germany
2
Institute of Veterinary Anatomy, Histology and Embryology, University of Leipzig, 04103 Leipzig, Germany
3
Rudolf-Boehm-Institute for Pharmacology and Toxicology, University of Leipzig, 04107 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Received: 26 January 2018 / Revised: 14 February 2018 / Accepted: 19 February 2018 / Published: 23 February 2018
(This article belongs to the Special Issue The Mechanism of Action of Food Components in Disease Prevention 2017)
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Abstract

Cardioplegic arrest during heart operations is often used in cardiac surgery. During cardioplegia, the heart is subjected to a global ischemia/reperfusion-injury. (−)-epigallocatechin gallate (EGCG), one of the main ingredients of green tea, seems to be beneficial in various cardiac diseases. Therefore, the aim of our study was to evaluate EGCG in a rabbit model of cardioplegic arrest. Twenty four mature Chinchilla rabbits were examined. Rabbit hearts were isolated and perfused according to Langendorff. After induction of cardioplegia (without and with 20 µmol/L EGCG, n = 6 each) the hearts maintained arrested for 90-min. Thereafter, the hearts were re-perfused for 60 min. During the entire experiment hemodynamic and functional data were assessed. At the end of each experiment, left ventricular samples were processed for ATP measurements and for histological analysis. Directly after cessation of cardioplegia, all hearts showed the same decline in systolic and diastolic function. However, hearts of the EGCG-group showed a significantly faster and better hemodynamic recovery during reperfusion. In addition, tissue ATP-levels were significantly higher in the EGCG-treated hearts. Histological analysis revealed that markers of nitrosative and oxidative stress were significantly lower in the EGCG group. Thus, addition of EGCG significantly protected the cardiac muscle from ischemia/reperfusion injury. View Full-Text
Keywords: EGCG; cardioplegia; ischemia/reperfusion injury; Langendorff EGCG; cardioplegia; ischemia/reperfusion injury; Langendorff
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Salameh, A.; Schuster, R.; Dähnert, I.; Seeger, J.; Dhein, S. Epigallocatechin Gallate Reduces Ischemia/Reperfusion Injury in Isolated Perfused Rabbit Hearts. Int. J. Mol. Sci. 2018, 19, 628.

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