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Int. J. Mol. Sci. 2018, 19(2), 568; https://doi.org/10.3390/ijms19020568

AP-1/KIF13A Blocking Peptides Impair Melanosome Maturation and Melanin Synthesis

1
Institut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, F-75005 Paris, France
2
Institut Curie, PSL Research University, CNRS, UMR144, Cell and Tissue Imaging Facility (PICT-IBiSA), F-75005 Paris, France
Present address: Technology Transfer and Industrial Partnerships Office, Institut Curie, F-75005 Paris, France.
Present address: Institut NeuroMyoGene (INMG), UCBL1, UMR 5310, INSERM U1217, Génétique et neurobiologie de C. Elegans, Faculté de Médecine et de Pharmacie, 8 Avenue Rockefeller, 69008 Lyon, France.
*
Author to whom correspondence should be addressed.
Received: 23 January 2018 / Revised: 7 February 2018 / Accepted: 9 February 2018 / Published: 14 February 2018
(This article belongs to the Special Issue Melanins and Melanogenesis: From Nature to Applications)
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Abstract

Melanocytes are specialized cells that generate unique organelles called melanosomes in which melanin is synthesized and stored. Melanosome biogenesis and melanocyte pigmentation require the transport and delivery of melanin synthesizing enzymes, such as tyrosinase and related proteins (e.g., TYRP1), from endosomes to maturing melanosomes. Among the proteins controlling endosome-melanosome transport, AP-1 together with KIF13A coordinates the endosomal sorting and trafficking of TYRP1 to melanosomes. We identify here β1-adaptin AP-1 subunit-derived peptides of 5 amino acids that block the interaction of KIF13A with AP-1 in cells. Incubating these peptides with human MNT-1 cells or 3D-reconstructed pigmented epidermis decreases pigmentation by impacting the maturation of melanosomes in fully pigmented organelles. This study highlights that peptides targeting the intracellular trafficking of melanocytes are candidate molecules to tune pigmentation in health and disease. View Full-Text
Keywords: pigmentation; melanocytes; kinesin; adaptor protein; peptide; melanosome biogenesis and maturation pigmentation; melanocytes; kinesin; adaptor protein; peptide; melanosome biogenesis and maturation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Campagne, C.; Ripoll, L.; Gilles-Marsens, F.; Raposo, G.; Delevoye, C. AP-1/KIF13A Blocking Peptides Impair Melanosome Maturation and Melanin Synthesis. Int. J. Mol. Sci. 2018, 19, 568.

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