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Open AccessArticle

Nano-Pore Size of Alumina Affects Osteoblastic Response

CIR Dental School, Department of Surgical Sciences, University of Turin, via Nizza 230, 10126 Turin, Italy
Department of Life Sciences and Systems Biology, UNITO, via Accademia Albertina 13, 10123 Turin, Italy
Department of Mechanical and Aerospatial Engineering (DIMEAS), Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy
Centre for Nanostructured Interfaces and Surfaces (NIS), via Quarello 11/A, 10135 Turin, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(2), 528;
Received: 19 January 2018 / Revised: 4 February 2018 / Accepted: 6 February 2018 / Published: 9 February 2018
The rapid development and application of nanotechnology to biological interfaces has impacted the bone implant field, allowing researchers to finely modulate the interface between biomaterials and recipient tissues. In the present study, oxidative anodization was exploited to generate two alumina surfaces with different pore diameters. The former displayed surface pores in the mean range of 16–30 nm, while in the latter pores varied from to 65 to 89 nm. The samples were characterized by Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray spectroscopy (EDX) analysis prior to being tested with pre-osteoblastic MC3T3-E1 cells. In vitro cell response was studied in terms of early cell adhesion, viability, and morphology, including focal adhesion quantification. Both the alumina samples promoted higher cell adhesion and viability than the control condition represented by the standard culture dish plastic. Osteogenic differentiation was assessed through alkaline phosphatase activity and extracellular calcium deposition, and it was found that of the two nano-surfaces, one was more efficient than the other. By comparing for the first time two nano-porous alumina surfaces with different pore diameters, our data supported the role of nano-topography in inducing cell response. Modulating a simple aspect of surface texture may become an attractive route for guiding bone healing and regeneration around implantable metals. View Full-Text
Keywords: MC3T3 cells; nano-porous alumina; nanotexture; cell adhesion; cell viability; in vitro osteogenesis MC3T3 cells; nano-porous alumina; nanotexture; cell adhesion; cell viability; in vitro osteogenesis
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MDPI and ACS Style

Mussano, F.; Genova, T.; Serra, F.G.; Carossa, M.; Munaron, L.; Carossa, S. Nano-Pore Size of Alumina Affects Osteoblastic Response. Int. J. Mol. Sci. 2018, 19, 528.

AMA Style

Mussano F, Genova T, Serra FG, Carossa M, Munaron L, Carossa S. Nano-Pore Size of Alumina Affects Osteoblastic Response. International Journal of Molecular Sciences. 2018; 19(2):528.

Chicago/Turabian Style

Mussano, Federico; Genova, Tullio; Serra, Francesca G.; Carossa, Massimo; Munaron, Luca; Carossa, Stefano. 2018. "Nano-Pore Size of Alumina Affects Osteoblastic Response" Int. J. Mol. Sci. 19, no. 2: 528.

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