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3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis
1
Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, Magurele, RO-077125 Bucharest, Romania
2
Faculty of Applied Sciences, University Politehnica of Bucharest, RO-060042 Bucharest, Romania
3
Horia Hulubei National Institute for Physics and Nuclear Engineering IFIN-HH, Magurele, RO-077125 Bucharest, Romania
4
Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, RO-011061 Bucharest, Romania
5
National Institute for Laser, Plasma and Radiation Physics, Magurele, RO-077125 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(2), 495; https://doi.org/10.3390/ijms19020495
Received: 27 December 2017 / Revised: 24 January 2018 / Accepted: 29 January 2018 / Published: 7 February 2018
(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering 2018)
We designed, fabricated and optimized 3D biomimetic magnetic structures that stimulate the osteogenesis in static magnetic fields. The structures were fabricated by direct laser writing via two-photon polymerization of IP-L780 photopolymer and were based on ellipsoidal, hexagonal units organized in a multilayered architecture. The magnetic activity of the structures was assured by coating with a thin layer of collagen-chitosan-hydroxyapatite-magnetic nanoparticles composite. In vitro experiments using MG-63 osteoblast-like cells for 3D structures with gradients of pore size helped us to find an optimum pore size between 20–40 µm. Starting from optimized 3D structures, we evaluated both qualitatively and quantitatively the effects of static magnetic fields of up to 250 mT on cell proliferation and differentiation, by ALP (alkaline phosphatase) production, Alizarin Red and osteocalcin secretion measurements. We demonstrated that the synergic effect of 3D structure optimization and static magnetic stimulation enhances the bone regeneration by a factor greater than 2 as compared with the same structure in the absence of a magnetic field.
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Keywords:
static magnetic field stimulation; 3D biomimetic structures; bone cell growth and differentiation
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MDPI and ACS Style
Paun, I.A.; Popescu, R.C.; Calin, B.S.; Mustaciosu, C.C.; Dinescu, M.; Luculescu, C.R. 3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis. Int. J. Mol. Sci. 2018, 19, 495.
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