Next Article in Journal
LncRNA HOTTIP-Mediated HOXA11 Expression Promotes Cell Growth, Migration and Inhibits Cell Apoptosis in Breast Cancer
Previous Article in Journal
Key Aspects of Nucleic Acid Library Design for in Vitro Selection
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(2), 471;

Importance of a Laccase Gene (Lcc1) in the Development of Ganoderma tsugae

College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Gutian 352200, China
Institute of Occean Science, Minjiang University, Fuzhou 350002, China
Authors to whom correspondence should be addressed.
Received: 31 December 2017 / Revised: 28 January 2018 / Accepted: 1 February 2018 / Published: 6 February 2018
(This article belongs to the Section Molecular Plant Sciences)
Full-Text   |   PDF [8565 KB, uploaded 6 February 2018]   |  


In this study, a novel laccase gene (Lcc1) from Ganoderma tsugae was isolated and its functions were characterized in detail. The results showed that Lcc1 has the highest expression activity during mycelium development and fruit body maturation based on the analysis of Lcc1 RNA transcripts at different developmental stages of G. tsugae. To investigate the exact contribution of Lcc1 to mycelium and fruit body development in G. tsugae, Lcc1 transgenic strains were constructed by targeted gene replacement and over-expression approaches. The results showed that the lignin degradation rate in Lcc1 deletion mutant was much lower than the degradation efficiency of the wild-type (WT), over-expression and rescue strains. The lignin degradation activity of G. tsugae is dependent on Lcc1 and the deletion of Lcc1 exerted detrimental influences on the development of mycelium branch. Furthermore, the study uncovered that Lcc1 deletion mutants generated much shorter pale grey fruit bodies, suggesting that Lcc1 contributes directly to pigmentation and stipe elongation during fruit body development in G. tsugae. The information obtained in this study provides a novel and mechanistic insight into the specific role of Lcc1 during growth and development of G. tsugae. View Full-Text
Keywords: Ganoderma tsugae; laccase; targeted gene replacement; over-expression; development Ganoderma tsugae; laccase; targeted gene replacement; over-expression; development

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Jin, W.; Li, J.; Feng, H.; You, S.; Zhang, L.; Norvienyeku, J.; Hu, K.; Sun, S.; Wang, Z. Importance of a Laccase Gene (Lcc1) in the Development of Ganoderma tsugae. Int. J. Mol. Sci. 2018, 19, 471.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top