Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye
AbstractA tumor vasculature network undergoes intense growth and rebuilding during tumor growth. Traditionally, vascular networks are histologically examined using parameters such as vessel density determined from two-dimensional slices of the tumor. Two-dimensional probing of a complicated three-dimensional (3D) structure only provides partial information. Therefore, we propose the use of microcomputed tomography (micro-CT) imaging to analyze the evolution of a tumor vasculature in an experimental ocular tumor model. A Bomirski Hamster Melanoma was implanted in the anterior chamber of a hamster eye. Ultrasound (US) imaging of the same tumor was performed in vivo, and the vascular results obtained using the two methods were compared. Normal ocular tissues, a tumor, and a tumor vascular structure were revealed with high accuracy using micro-CT. The vessels that grew within the tumor were chaotic, leaky, and contained many convoluted micro-vessels and embolizations. They comprised 20–38% of the tumor mass. The blood flow in the larger functional vessels was in the range from 10 to 25 mm/s, as determined by in vivo Doppler US. The micro-CT imaging of the hamster eyeball enabled both qualitative and quantitative 3D analyses of the globe at a histological level. Although the presented images were obtained ex vivo, micro-CT noninvasive imaging is being developed intensively, and high-resolution in vivo imaging is feasible. View Full-Text
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Leszczyński, B.; Śniegocka, M.; Wróbel, A.; Pędrys, R.; Szczygieł, M.; Romanowska-Dixon, B.; Urbańska, K.; Elas, M. Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye. Int. J. Mol. Sci. 2018, 19, 332.
Leszczyński B, Śniegocka M, Wróbel A, Pędrys R, Szczygieł M, Romanowska-Dixon B, Urbańska K, Elas M. Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye. International Journal of Molecular Sciences. 2018; 19(2):332.Chicago/Turabian Style
Leszczyński, Bartosz; Śniegocka, Martyna; Wróbel, Andrzej; Pędrys, Roman; Szczygieł, Małgorzata; Romanowska-Dixon, Bożena; Urbańska, Krystyna; Elas, Martyna. 2018. "Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye." Int. J. Mol. Sci. 19, no. 2: 332.
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