Stimulation of Peritoneal Mesothelial Cells to Secrete Matrix Metalloproteinase-9 (MMP-9) by TNF-α: A Role in the Invasion of Gastric Carcinoma Cells
Abstract
:1. Introduction
2. Results
2.1. Mesothelial Cells Secreted MMP-9 in Response to Stimulation with TNF-α
2.2. TNF-α Potentiates MKN1 Cell Invasion through the Reconstituted Mesothelium
2.3. The Exogenous Addition of MMP-9 Promotes MKN1 Cell Invasion
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cells
4.3. Gelatin Zymography
4.4. Quantitative RT-PCR (RT-qPCR)
4.5. Cell Invasion Assay Using Reconstituted Mesothelium
4.6. Cell Adhesion Assay
4.7. Flow Cytometry
4.8. MMP-Knockdown MKN1 Cells
4.9. Purification of MMP-9
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BCECF-AM | 3′-O-acetyl-2′,7′-bis(carboxyethyl)-4 or 5-carboxyfluorescein, diacetoxymethyl ester |
CBB | Coomassie brilliant blue |
ECM | extracellular matrix |
FCS | fetal calf serum |
MALDI/TOFMS | matrix-assisted laser desorption ionization/time-of-flight mass spectrometer |
MMP | matrix metalloproteinase |
PBS | phosphate-buffered saline |
PMA | phorbol 12-myristate 13-acetate |
RT-PCR | reverse transcription-polymerase chain reaction |
TNF-α | tumor necrosis factor-α |
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Oku, T.; Shimada, K.; Kenmotsu, H.; Ando, Y.; Kurisaka, C.; Sano, R.; Tsuiji, M.; Hasegawa, S.; Fukui, T.; Tsuji, T. Stimulation of Peritoneal Mesothelial Cells to Secrete Matrix Metalloproteinase-9 (MMP-9) by TNF-α: A Role in the Invasion of Gastric Carcinoma Cells. Int. J. Mol. Sci. 2018, 19, 3961. https://doi.org/10.3390/ijms19123961
Oku T, Shimada K, Kenmotsu H, Ando Y, Kurisaka C, Sano R, Tsuiji M, Hasegawa S, Fukui T, Tsuji T. Stimulation of Peritoneal Mesothelial Cells to Secrete Matrix Metalloproteinase-9 (MMP-9) by TNF-α: A Role in the Invasion of Gastric Carcinoma Cells. International Journal of Molecular Sciences. 2018; 19(12):3961. https://doi.org/10.3390/ijms19123961
Chicago/Turabian StyleOku, Teruaki, Kentaro Shimada, Hiroki Kenmotsu, Yusuke Ando, Chisato Kurisaka, Rikio Sano, Makoto Tsuiji, Shinya Hasegawa, Tetsuya Fukui, and Tsutomu Tsuji. 2018. "Stimulation of Peritoneal Mesothelial Cells to Secrete Matrix Metalloproteinase-9 (MMP-9) by TNF-α: A Role in the Invasion of Gastric Carcinoma Cells" International Journal of Molecular Sciences 19, no. 12: 3961. https://doi.org/10.3390/ijms19123961