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Int. J. Mol. Sci. 2018, 19(12), 3923; https://doi.org/10.3390/ijms19123923

Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B

1
Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
2
Department of Biology, Waldorf University, Forest City, IA 50436, USA
3
Department of Periodontology, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN 38103, USA
4
Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
5
College of Dentistry, University of Nebraska Medical Center, Lincoln, NE 68583, USA
6
Center for Molecular Microbiology and Department of Oral Biology, University of Florida, Gainesville, FL 32603, USA
*
Author to whom correspondence should be addressed.
Received: 20 November 2018 / Revised: 30 November 2018 / Accepted: 4 December 2018 / Published: 7 December 2018
(This article belongs to the Special Issue Matrix Metalloproteinase)
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Abstract

Matrix metalloproteinases (MMPs) are enzymes involved in periodontal tissue destruction. Hemagglutinin B (HagB) from the periodontal pathogen Porphyromonas gingivalis induces an elevated MMP response in dendritic cells, but responses from cultures of single-cell types do not reflect the local tissue environment. The objective of this study was to measure HagB-induced MMP responses in a transwell co-culture system containing dendritic cells, gingival epithelial (GE) keratinocytes, and CD4+ T-cells. Transwell co-cultures were assembled and treated with or without HagB. Immunoassays were used to determine production of MMP1, MMP7, MMP9, and MMP12 in response to HagB up to 64 h. Control responses were subtracted from HagB-induced responses. A two-way fixed effect ANOVA was fit to log-transformed concentrations and pairwise group comparisons were conducted (p < 0.05). At 64 h, dendritic cells produced elevated MMP1 and MMP9 responses, which were attenuated in the 3-cell co-culture (p < 0.05). There were also significant differences in MMP7 and MMP12 production between single-cell cultures and co-cultures. These results support the need to use multiple cell types in culture models to evaluate a more representative response to proinflammatory agonists. This three-cell transwell co-culture model may help us better understand the inflammatory process in periodontal disease and test novel therapeutic approaches. View Full-Text
Keywords: hemagglutinin-B; transwell co-cultures; matrix metalloproteinases hemagglutinin-B; transwell co-cultures; matrix metalloproteinases
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Bates, A.M.; Fischer, C.L.; Abhyankar, V.P.; Johnson, G.K.; Guthmiller, J.M.; Progulske-Fox, A.; Brogden, K.A. Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B. Int. J. Mol. Sci. 2018, 19, 3923.

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