Next Article in Journal
Drosophila as a Model to Study Brain Innate Immunity in Health and Disease
Next Article in Special Issue
Nutrient-Responsive Small Signaling Peptides and Their Influence on the Root System Architecture
Previous Article in Journal
MET/HGF Co-Targeting in Pancreatic Cancer: A Tool to Provide Insight into the Tumor/Stroma Crosstalk
Previous Article in Special Issue
Adaptation Mechanism of Salt Excluders under Saline Conditions and Its Applications
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(12), 3921; https://doi.org/10.3390/ijms19123921

LeNRT1.1 Improves Nitrate Uptake in Grafted Tomato Plants under High Nitrogen Demand

1
Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4860, Macul, Santiago 7820436, Chile
2
Centro UC Desierto de Atacama, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4860, Macul, Santiago 7820436, Chile
3
Departamento de Producción Agrícola, Facultad de Ciencia Agrarias, Universidad de Talca, Avenida Lircay S/N, P.O. Box 747, Talca 3462227, Chile
*
Author to whom correspondence should be addressed.
Received: 23 October 2018 / Revised: 3 December 2018 / Accepted: 3 December 2018 / Published: 7 December 2018
Full-Text   |   PDF [2053 KB, uploaded 7 December 2018]   |  

Abstract

Grafting has become a common practice among tomato growers to obtain vigorous plants. These plants present a substantial increase in nitrogen (N) uptake from the root zone. However, the mechanisms involved in this higher uptake capacity have not been investigated. To elucidate whether the increase in N uptake in grafted tomato plants under high N demand conditions is related to the functioning of low- (high capacity) or high-affinity (low capacity) root plasma membrane transporters, a series of experiments were conducted. Plants grafted onto a vigorous rootstock, as well as ungrafted and homograft plants, were exposed to two radiation levels (400 and 800 µmol m−2 s−1). We assessed root plasma membrane nitrate transporters (LeNRT1.1, LeNRT1.2, LeNRT2.1, LeNRT2.2 and LeNRT2.3) expression, Michaelis‒Menten kinetics parameters (Vmax and Km), root and leaf nitrate reductase activity, and root respiration rates. The majority of nitrate uptake is mediated by LeNRT1.1 and LeNRT1.2 in grafted and ungrafted plants. Under high N demand conditions, vigorous rootstocks show similar levels of expression for LeNRT1.1 and LeNRT1.2, whereas ungrafted plants present a higher expression of LeNRT1.2. No differences in the uptake capacity (evaluated as Vmax), root respiration rates, or root nitrate assimilation capacity were found among treatments. View Full-Text
Keywords: nitrate transporters expression; growth rate; root membrane transporters; root respiration nitrate transporters expression; growth rate; root membrane transporters; root respiration
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Albornoz, F.; Gebauer, M.; Ponce, C.; Cabeza, R.A. LeNRT1.1 Improves Nitrate Uptake in Grafted Tomato Plants under High Nitrogen Demand. Int. J. Mol. Sci. 2018, 19, 3921.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top