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Int. J. Mol. Sci. 2018, 19(12), 3916; https://doi.org/10.3390/ijms19123916

MicroRNA 210 Mediates VEGF Upregulation in Human Periodontal Ligament Stem Cells Cultured on 3DHydroxyapatite Ceramic Scaffold

1
Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, Italy
2
ASL02 Lanciano-Vasto-Chieti, Chieti “Ss. Annunziata” Hospital, 66100 Chieti, Italy
3
Faculté de Médecine, UMR 7365 CNRS-Université de Lorraine, 9, Avenue de la Forêt de Haye, 54500 Vandoeuvre-lés-Nancy, France
4
Laser in Dentistry Program, Cruzeiro do Sul University (UNICSUL), 08060-070 Sao Paulo-SP, Brazil
*
Author to whom correspondence should be addressed.
Received: 23 November 2018 / Revised: 3 December 2018 / Accepted: 5 December 2018 / Published: 6 December 2018
(This article belongs to the Special Issue Role and Application of Stem Cells in Regenerative Medicine)
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Abstract

The aim of the present research was the evaluation of the behavior of human periodontal ligament stem cells (hPDLSCs), cultured in presence of Endobon® Xenograft Granules (G), a fully deproteinated hydroxyapatite ceramic scaffold derived from cancellous bovine bone. hPDLSCs were seeded with and without G for 24 h to 1 week. The cell growth, morphological features, adhesiveness, differentiation ability, modulation of miR-210 and Vascular Endothelial Growth Factor (VEGF) secretion were analyzed by means of MTT assay, Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), Alizarin Red S assay, RT-PCR and ELISA test, respectively. hPDLSCs grown on the biomaterial showed the ability to form focal adhesion on the substrate, as demonstrated by vinculin expression. These data were supported by SEM analysis showing that an adhesiveness process associated to cell growth occurs between cells and biomaterials. The osteogenic differentiation, evaluated by morphological, biochemical, and RT-PCR analysis, was pronounced in the hPDLSCs grown in the three-dimensional inorganic bovine bone substitute in the presence of osteoinductive conditions. In addition, an upregulation of miR-210 and VEGF was evident in cells cultured in presence of the biomaterial. Our results inspire us to consider granules not only an adequate biocompatible three-dimensional biomaterial, but also an effective inductor of miR-210 and VEGF; in fact, the involvement of miR-210 in VEGF secretion could offer a novel regulatory system in the early steps of the bone-regeneration process. View Full-Text
Keywords: human periodontal ligament stem cells; bone substitutes; vascular endothelial growth factors; miR-210; osteogenesis human periodontal ligament stem cells; bone substitutes; vascular endothelial growth factors; miR-210; osteogenesis
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Pizzicannella, J.; Cavalcanti, M.; Trubiani, O.; Diomede, F. MicroRNA 210 Mediates VEGF Upregulation in Human Periodontal Ligament Stem Cells Cultured on 3DHydroxyapatite Ceramic Scaffold. Int. J. Mol. Sci. 2018, 19, 3916.

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