Next Article in Journal
Advancement of NF-κB Signaling Pathway: A Novel Target in Pancreatic Cancer
Previous Article in Journal
Morphological Characterization of Root System Architecture in Diverse Tomato Genotypes during Early Growth
Article

Heterologous Expression of the Grapevine JAZ7 Gene in Arabidopsis Confers Enhanced Resistance to Powdery Mildew but Not to Botrytis cinerea

by 1,2,†, 1,2,†, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China
2
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling 712100, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(12), 3889; https://doi.org/10.3390/ijms19123889
Received: 27 October 2018 / Revised: 25 November 2018 / Accepted: 30 November 2018 / Published: 5 December 2018
(This article belongs to the Section Molecular Plant Sciences)
Jasmonate ZIM-domain (JAZ) family proteins comprise a class of transcriptional repressors that silence jasmonate-inducible genes. Although a considerable amount of research has been carried out on this gene family, there is still very little information available on the role of specific JAZ gene members in multiple pathogen resistance, especially in non-model species. In this study, we investigated the potential resistance function of the VqJAZ7 gene from a disease-resistant wild grapevine, Vitis quinquangularis cv. “Shang-24”, through heterologous expression in Arabidopsis thaliana. VqJAZ7-expressing transgenic Arabidopsis were challenged with three pathogens: the biotrophic fungus Golovinomyces cichoracearum, necrotrophic fungus Botrytis cinerea, and semi-biotrophic bacteria Pseudomonas syringae pv. tomato DC3000. We found that plants expressing VqJAZ7 showed greatly reduced disease symptoms for G. cichoracearum, but not for B. cinerea or P. syringae. In response to G cichoracearum infection, VqJAZ7-expressing transgenic lines exhibited markedly higher levels of cell death, superoxide anions (O2¯, and H2O2 accumulation, relative to nontransgenic control plants. Moreover, we also tested the relative expression of defense-related genes to comprehend the possible induced pathways. Taken together, our results suggest that VqJAZ7 in grapevine participates in molecular pathways of resistance to G. cichoracearum, but not to B. cinerea or P. syringae. View Full-Text
Keywords: VqJAZ7; grapevine; Golovinomyces cichoracearum; powdery mildew; Botrytis cinerea; Pst DC3000 VqJAZ7; grapevine; Golovinomyces cichoracearum; powdery mildew; Botrytis cinerea; Pst DC3000
Show Figures

Figure 1

MDPI and ACS Style

Hanif, M.; Rahman, M.U.; Gao, M.; Yang, J.; Ahmad, B.; Yan, X.; Wang, X. Heterologous Expression of the Grapevine JAZ7 Gene in Arabidopsis Confers Enhanced Resistance to Powdery Mildew but Not to Botrytis cinerea. Int. J. Mol. Sci. 2018, 19, 3889. https://doi.org/10.3390/ijms19123889

AMA Style

Hanif M, Rahman MU, Gao M, Yang J, Ahmad B, Yan X, Wang X. Heterologous Expression of the Grapevine JAZ7 Gene in Arabidopsis Confers Enhanced Resistance to Powdery Mildew but Not to Botrytis cinerea. International Journal of Molecular Sciences. 2018; 19(12):3889. https://doi.org/10.3390/ijms19123889

Chicago/Turabian Style

Hanif, Muhammad; Rahman, Mati U.; Gao, Min; Yang, Jinhua; Ahmad, Bilal; Yan, Xiaoxiao; Wang, Xiping. 2018. "Heterologous Expression of the Grapevine JAZ7 Gene in Arabidopsis Confers Enhanced Resistance to Powdery Mildew but Not to Botrytis cinerea" Int. J. Mol. Sci. 19, no. 12: 3889. https://doi.org/10.3390/ijms19123889

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop