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Int. J. Mol. Sci. 2018, 19(11), 3504; https://doi.org/10.3390/ijms19113504

Pediococcus pentosaceus-Fermented Cordyceps militaris Inhibits Inflammatory Reactions and Alleviates Contact Dermatitis

1
Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea
2
Department of, College of Food Biotechnology, Division of Bioindustry, Silla University, Busan 46958, Korea
3
Department of Life Science, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea
4
Department of BioNano Technology, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, Korea
The first two authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 August 2018 / Revised: 3 October 2018 / Accepted: 5 November 2018 / Published: 7 November 2018
(This article belongs to the Special Issue NF-κB and Cancer)
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Abstract

Cordyceps militaris is a medicinal mushroom used to treat immune-related diseases in East Asia. We investigated the anti-inflammatory effect of the extract of C. militaris grown on germinated Rhynchosia nulubilis (GRC) fermented with Pediococcus pentosaceus ON89A isolated from onion (GRC-ON89A) in vivo as well as in vitro. The anti-inflammatory effect of GRC-ON89A was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The total polyphenol content (TPC) and total flavonoid content (TFC) in the GRC-ON89A ethanol extract were significantly increased compared to that in GRC. GRC-ON89A hexane fraction (GRC-ON89A-Hex) inhibited the release of nitric oxide (NO) compared to that of the LPS-treated control without cytotoxicity in LPS-stimulated RAW 264.7 macrophages. GRC-ON89A-Hex decreased the inducible NO synthase (iNOS), cyclooxygenase 2 (COX2), and tumor necrosis factor (TNF)-α mRNA expression in LPS-stimulated RAW 264.7 macrophages. In addition, pre-treatment with GRC-ON89A-Hex significantly inhibited LPS-stimulated phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB. To induce allergic contact dermatitis (ACD), 1-fluoro-2, 4-dinitrofluorobenzene (DNFB) was applied to the surface of the right ears of C57BL/6N mice. GRC-ON89A reduced the ear swelling and thickness in DNFB-induced ACD mice. This study demonstrates the potential usefulness of GRC-ON89A as an anti-inflammatory dietary supplement or drug. View Full-Text
Keywords: GRC-ON89A; macrophage; anti-inflammatory; DNFB-induced ACD mice; NF-κB GRC-ON89A; macrophage; anti-inflammatory; DNFB-induced ACD mice; NF-κB
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Kwon, H.-K.; Song, M.-J.; Lee, H.-J.; Park, T.-S.; Kim, M.I.; Park, H.-J. Pediococcus pentosaceus-Fermented Cordyceps militaris Inhibits Inflammatory Reactions and Alleviates Contact Dermatitis. Int. J. Mol. Sci. 2018, 19, 3504.

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