Characterization of Hydroxyproline-Containing Hairpin-Like Antimicrobial Peptide EcAMP1-Hyp from Barnyard Grass (Echinochloa crusgalli L.) Seeds: Structural Identification and Comparative Analysis of Antifungal Activity
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Biological Material
4.2. Isolation of Hyp-Containing EcAMP1 from E. crusgalli Seeds
4.3. Mass Spectrometry
4.4. N-Terminal Sequencing
4.5. Peptide Synthesis
4.6. Obtainment of the Recombinant EcAMP1 in the E. coli System
4.7. Chitin- and β-1.3-Glucan-Binding Assay In Vitro
4.8. Molecular Modeling
4.9. Antifungal Activity
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Peptide | ICmin | IC50 |
---|---|---|
EcAMP1-Hyp | 2.9 | 5.4 |
EcAMP1 | 2.2 | 3.8 |
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Rogozhin, E.; Zalevsky, A.; Mikov, A.; Smirnov, A.; Egorov, T. Characterization of Hydroxyproline-Containing Hairpin-Like Antimicrobial Peptide EcAMP1-Hyp from Barnyard Grass (Echinochloa crusgalli L.) Seeds: Structural Identification and Comparative Analysis of Antifungal Activity. Int. J. Mol. Sci. 2018, 19, 3449. https://doi.org/10.3390/ijms19113449
Rogozhin E, Zalevsky A, Mikov A, Smirnov A, Egorov T. Characterization of Hydroxyproline-Containing Hairpin-Like Antimicrobial Peptide EcAMP1-Hyp from Barnyard Grass (Echinochloa crusgalli L.) Seeds: Structural Identification and Comparative Analysis of Antifungal Activity. International Journal of Molecular Sciences. 2018; 19(11):3449. https://doi.org/10.3390/ijms19113449
Chicago/Turabian StyleRogozhin, Eugene, Artur Zalevsky, Alexander Mikov, Alexey Smirnov, and Tsezi Egorov. 2018. "Characterization of Hydroxyproline-Containing Hairpin-Like Antimicrobial Peptide EcAMP1-Hyp from Barnyard Grass (Echinochloa crusgalli L.) Seeds: Structural Identification and Comparative Analysis of Antifungal Activity" International Journal of Molecular Sciences 19, no. 11: 3449. https://doi.org/10.3390/ijms19113449