Next Article in Journal
Hepatocyte Growth Factor Activator: A Proteinase Linking Tissue Injury with Repair
Next Article in Special Issue
Original Macromolecular Architectures Based on poly(ε-caprolactone) and poly(ε-thiocaprolactone) Grafted onto Chitosan Backbone
Previous Article in Journal
The Antitumor Natural Compound Falcarindiol Disrupts Neural Stem Cell Homeostasis by Suppressing Notch Pathway
Previous Article in Special Issue
Chemo-Enzymatic Synthesis of Renewable Sterically-Hindered Phenolic Antioxidants with Tunable Polarity from Lignocellulose and Vegetal Oil Components
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(11), 3433; https://doi.org/10.3390/ijms19113433

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

1
Institute of Clinical Experimental Research, Semmelweis University, 1094 Budapest, Hungary
2
Orthosera GmbH, 3500 Krems an der Donau, Austria
3
Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, 1111 Budapest, Hungary
4
Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences,1117 Budapest, Hungary
5
Department of Polymer Engineering, Faculty of Mechanical Engineering, Budapest University of Technology and Economics, 1111 Budapest, Hungary
6
Center for Regenerative Medicine, Danube University, 3500 Krems-an-der-Donau, Austria
7
Institute of Sport and Health Sciences, University of Physical Education, 1123 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Received: 28 September 2018 / Revised: 24 October 2018 / Accepted: 29 October 2018 / Published: 1 November 2018
(This article belongs to the Special Issue Biobased and/or Biodegradable Polymeric Materials)
Full-Text   |   PDF [3724 KB, uploaded 1 November 2018]   |  

Abstract

Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It is widely used in soft and hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling of the membranes, it degrades easily, and it has a low tensile strength which does not hold a suture blocking wider clinical applications of PRF. Our aim was to produce a sterile, suturable, reproducible PRF membrane suitable for surgical intervention. We compared the biological and mechanical properties of PRF membranes created by the classical glass-tube and those that were created in a single-syringe closed system (hypACT Inject), which allowed aseptic preparation. HypACT Inject device produces a PRF membrane with better handling characteristics without compromising biological properties. Freeze-thawing resulted in significantly higher tensile strength and higher cell adhesion at a lower degradation rate of the membranes. Mesenchymal stem cells seeded onto PRF membranes readily proliferated on the surface of fresh, but even better on freeze/thawed or freeze-dried membranes. These data show that PRF membranes can be made sterile, more uniform and significantly stronger which makes it possible to use them as suturable surgical membranes. View Full-Text
Keywords: biopolymer; platelet-rich fibrin; scaffold; biodegradation; tissue regeneration; medical device biopolymer; platelet-rich fibrin; scaffold; biodegradation; tissue regeneration; medical device
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Kardos, D.; Hornyák, I.; Simon, M.; Hinsenkamp, A.; Marschall, B.; Várdai, R.; Kállay-Menyhárd, A.; Pinke, B.; Mészáros, L.; Kuten, O.; Nehrer, S.; Lacza, Z. Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System. Int. J. Mol. Sci. 2018, 19, 3433.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top