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Inhibitory and Inductive Effects of Opuntia ficus indica Extract and Its Flavonoid Constituents on Cytochrome P450s and UDP-Glucuronosyltransferases

1
Department of Biological Sciences, Graduate School of Sookmyung Women’s University, Seoul 04310, Korea
2
Department of Biology Education, College of Education, Sookmyung Women’s University, Seoul 04310, Korea
3
Department of Pharmacy, College of Pharmacy, Sookmyung Women’s University, Seoul 04310, Korea
4
Department of Biopharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA
5
Department of Biological Sciences, Research Institute for Women’s Health, Sookmyung Women’s University, Seoul 04310, Korea
*
Authors to whom correspondence should be addressed.
These two authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(11), 3400; https://doi.org/10.3390/ijms19113400
Received: 8 September 2018 / Revised: 18 October 2018 / Accepted: 26 October 2018 / Published: 30 October 2018
(This article belongs to the Special Issue Polyphenols: Nutrition, Physiology, Metabolism and Health Benefits)
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Abstract

Opuntia ficus indica (OFI) is grown abundantly in arid areas and its fruits are regarded as an important food and nutrient source owing to the presence of flavonoids, minerals, and proteins. The previous report that OFI exerts phytoestrogenic activity makes it plausible for OFI-containing supplements to be used as alternative estrogen replacement therapy. In the case of polypharmacy with the consumption of OFI-containing botanicals in post- or peri-menopausal women, it is critical to determine the potential drug-OFI interaction due to the modulation of drug metabolism. In the present study, the modulating effects on the hepatic drug metabolizing enzymes (DMEs) by OFI and its flavonoid constituents (kaempferol, quercetin, isorhamnetin, and their glycosidic forms) were investigated using the liver microsomal fractions prepared from ovariectomized (OVX) rats, human liver microsomes, and human hepatocarcinoma cell line (HepG2). As a result, the oral administration of extracts of OFI (OFIE) in OVX rats induced hepatic CYP2B1, CYP3A1, and UGT2B1. OFIE, hydrolyzed (hdl) OFIE, and several flavonols induced the transcriptional activities of both CYP2B6 and CYP3A4 genes in HepG2 cells. Finally, OFIE did not inhibit activities of cytochrome P450 (CYPs) or uridine diphosphate (UDP)-glucuronosyltransferases (UGTs), whereas hdl OFIE or flavonol treatment inhibited CYP1A2 and CYP3A1/3A4 in rat and human liver microsomes. Our data demonstrate that OFIE may induce or inhibit certain types of DMEs and indicate that drug-OFI interaction may occur when the substrate or inhibitor drugs of specific CYPs or UGTs are taken concomitantly with OFI-containing products. View Full-Text
Keywords: Opuntia ficus indica; flavonoids; drug metabolism; cytochrome P450; uridine diphosphate-glucuronosyltransferase; drug-herb interactions; menopausal women Opuntia ficus indica; flavonoids; drug metabolism; cytochrome P450; uridine diphosphate-glucuronosyltransferase; drug-herb interactions; menopausal women
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jeong, H.; Kim, S.; Kim, M.-Y.; Lee, J.; An, B.H.; Kim, H.-D.; Jeong, H.; Song, Y.S.; Chang, M. Inhibitory and Inductive Effects of Opuntia ficus indica Extract and Its Flavonoid Constituents on Cytochrome P450s and UDP-Glucuronosyltransferases. Int. J. Mol. Sci. 2018, 19, 3400.

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