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Open AccessArticle

Genome-Wide Identification and Characterization of Pectin Methylesterase Inhibitor Genes in Brassica oleracea

by Tingting Liu 1,2,3, Hui Yu 1,2,3, Xingpeng Xiong 1,2,3, Youjian Yu 4, Xiaoyan Yue 1,2,3, Jinlong Liu 5 and Jiashu Cao 1,2,3,*
1
Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China
2
Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China
3
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, China
4
Department of Horticulture, College of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, China
5
Laboratory of Molecular Biology and Gene Engineering, School of Life Sciences, Nanchang University, Nanchang 330031, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(11), 3338; https://doi.org/10.3390/ijms19113338
Received: 5 September 2018 / Revised: 11 October 2018 / Accepted: 23 October 2018 / Published: 26 October 2018
(This article belongs to the Section Molecular Plant Sciences)
The activities of pectin methylesterases (PMEs) are regulated by pectin methylesterase inhibitors (PMEIs), which consequently control the pectin methylesterification status. However, the role of PMEI genes in Brassica oleracea, an economically important vegetable crop, is poorly understood. In this study, 95 B. oleracea PMEI (BoPMEI) genes were identified. A total of 77 syntenic ortholog pairs and 10 tandemly duplicated clusters were detected, suggesting that the expansion of BoPMEI genes was mainly attributed to whole-genome triplication (WGT) and tandem duplication (TD). During diploidization after WGT, BoPMEI genes were preferentially retained in accordance with the gene balance hypothesis. Most homologous gene pairs experienced purifying selection with ω (Ka/Ks) ratios lower than 1 in evolution. Five stamen-specific BoPMEI genes were identified by expression pattern analysis. By combining the analyses of expression and evolution, we speculated that nonfunctionalization, subfunctionalization, neofunctionalization, and functional conservation can occur in the long evolutionary process. This work provides insights into the characterization of PMEI genes in B. oleracea and contributes to the further functional studies of BoPMEI genes. View Full-Text
Keywords: Brassica oleracea; BoPMEI genes; whole-genome triplication; evolution; expression profiles Brassica oleracea; BoPMEI genes; whole-genome triplication; evolution; expression profiles
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Liu, T.; Yu, H.; Xiong, X.; Yu, Y.; Yue, X.; Liu, J.; Cao, J. Genome-Wide Identification and Characterization of Pectin Methylesterase Inhibitor Genes in Brassica oleracea. Int. J. Mol. Sci. 2018, 19, 3338.

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