Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
Abstract
:1. Introduction
2. Results
2.1. Sequencing of Small RNAs in Maize Roots
2.2. Identification of Known Maize miRNAs
2.3. Prediction of Novel miRNAs
2.4. Differentially Expressed miRNA (DEM) Responsive to Arbuscular Mycorrhiza (AM) Fungus
2.5. Prediction of the Differentially Expressed miRNAs Target Genes
2.6. Function and Enrichment Analyses of the Target Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Detection of Mycorrhizal Colonization
4.3. RNA Extraction, Small RNA Library Preparation and Sequencing
4.4. Identification of Known miRNAs and Novel miRNAs
4.5. Identification of AM Fungus Responsive miRNAs
4.6. Stem-Loop Reverse Transcription Polymerase Chain Reaction (RT-qPCR)
4.7. Identification of miRNA Targets
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Xu, Y.; Zhu, S.; Liu, F.; Wang, W.; Wang, X.; Han, G.; Cheng, B. Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize. Int. J. Mol. Sci. 2018, 19, 3201. https://doi.org/10.3390/ijms19103201
Xu Y, Zhu S, Liu F, Wang W, Wang X, Han G, Cheng B. Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize. International Journal of Molecular Sciences. 2018; 19(10):3201. https://doi.org/10.3390/ijms19103201
Chicago/Turabian StyleXu, Yunjian, Suwen Zhu, Fang Liu, Wei Wang, Xuewen Wang, Guomin Han, and Beijiu Cheng. 2018. "Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize" International Journal of Molecular Sciences 19, no. 10: 3201. https://doi.org/10.3390/ijms19103201