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The Tissue-Engineered Human Psoriatic Skin Substitute: A Valuable In Vitro Model to Identify Genes with Altered Expression in Lesional Psoriasis

1
Centre LOEX de l’Université Laval, Génie Tissulaire et Régénération, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC G1J 1Z4, Canada
2
Faculté de Pharmacie, Université Laval, Québec, QC G1V 0A6, Canada
3
Centre Universitaire d’Ophtalmologie-Recherche, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC G1S4L8, Canada
4
Département d’Ophtalmologie, Université Laval, Québec, QC G1V 0A6, Canada
5
Département de Dermatologie, Hôpital de l’Enfant-Jésus, Québec, QC G1J 1Z4, Canada
*
Author to whom correspondence should be addressed.
These two authors contributed equally to the work and therefore should be considered as equivalent first authors.
Int. J. Mol. Sci. 2018, 19(10), 2923; https://doi.org/10.3390/ijms19102923
Received: 4 September 2018 / Accepted: 16 September 2018 / Published: 26 September 2018
(This article belongs to the Special Issue Molecular Mechanisms and Pathology of Psoriasis)
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Abstract

Psoriasis is a chronic inflammatory skin disease for which no cure has emerged. Its complex etiology requires the development of an in vitro model representative of the pathology. In this study, we exploited gene profiling analyses on microarray in order to characterize and further optimize the production of a human psoriatic skin model representative of this in vivo skin disease. Various skin substitutes were produced by tissue-engineering using biopsies from normal, healthy donors, or from lesional or non-lesional skin samples from patients with psoriasis, and their gene expression profiles were examined by DNA microarray. We demonstrated that more than 3540 and 1088 genes (two-fold change) were deregulated between healthy/lesional and lesional/non-lesional psoriatic substitutes, respectively. Moreover, several genes related to lipid metabolism, such as PLA2G4E and PLA2G4C, were identified as repressed in the lesional substitutes. In conclusion, gene profiling analyses identified a list of deregulated candidate genes associated with various metabolic pathways that may contribute to the progression of psoriasis. View Full-Text
Keywords: psoriasis; gene profiling; tissue-engineering psoriasis; gene profiling; tissue-engineering
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Rioux, G.; Pouliot-Bérubé, C.; Simard, M.; Benhassine, M.; Soucy, J.; Guérin, S.L.; Pouliot, R. The Tissue-Engineered Human Psoriatic Skin Substitute: A Valuable In Vitro Model to Identify Genes with Altered Expression in Lesional Psoriasis. Int. J. Mol. Sci. 2018, 19, 2923.

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