Skin Protective Effect of Epigallocatechin Gallate
1
Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Korea
2
Heritage Material Research Team, Amorepacific Research and Development Unit, Yongin 17074, Korea
*
Authors to whom correspondence should be addressed.
†
There authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(1), 173; https://doi.org/10.3390/ijms19010173
Received: 26 December 2017
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Revised: 3 January 2018
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Accepted: 4 January 2018
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Published: 6 January 2018
(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2017)
Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase (HAS) and hyaluronidase (HYAL) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-PCR showed that EGCG increased the expression of natural moisturizing factor-related genes filaggrin (FLG), transglutaminase-1, HAS-1, and HAS-2. Under UVB irradiation conditions, the expression level of HYAL was decreased in HaCaT cells. Furthermore, we confirmed the antioxidant activity of EGCG and also showed a preventive effect against radical-evoked apoptosis by downregulation of caspase-8 and -3 in HaCaT cells. EGCG reduced melanin secretion and production in melanoma cells. Together, these results suggest that EGCG might be used as a cosmetic ingredient with positive effects on skin hydration, moisture retention, and wrinkle formation, in addition to radical scavenging activity and reduction of melanin generation.
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MDPI and ACS Style
Kim, E.; Hwang, K.; Lee, J.; Han, S.Y.; Kim, E.-M.; Park, J.; Cho, J.Y. Skin Protective Effect of Epigallocatechin Gallate. Int. J. Mol. Sci. 2018, 19, 173. https://doi.org/10.3390/ijms19010173
AMA Style
Kim E, Hwang K, Lee J, Han SY, Kim E-M, Park J, Cho JY. Skin Protective Effect of Epigallocatechin Gallate. International Journal of Molecular Sciences. 2018; 19(1):173. https://doi.org/10.3390/ijms19010173
Chicago/Turabian StyleKim, Eunji, Kyeonghwan Hwang, Jongsung Lee, Sang Y. Han, Eun-Mi Kim, Junseong Park, and Jae Y. Cho. 2018. "Skin Protective Effect of Epigallocatechin Gallate" International Journal of Molecular Sciences 19, no. 1: 173. https://doi.org/10.3390/ijms19010173
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