Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)
Abstract
:1. Introduction
2. Results
2.1. Phenotype of Premature Senescence Leaf Mutants
2.2. Alterations of Photosynthetic and Senescence-related Parameters
2.3. Impaired Chloroplast Development in the Mutants
2.4. Premature Senescence Leaf phenotypes Are Controlled by Single Recessive Genes
2.5. Darkness and ABA Induce Leaf Senescence
2.6. ROS Accumulation, Cell Death and DNA Fragmentation Occur in the Mutants
2.7. Alterations of ABA Contents and ABA-Related Gene Expression
2.8. Differential Expression of Genes Associated with Senescence, Chlorophyll Metabolism and Photosynthesis
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Genetic Analysis and Allelism Tests
4.3. Histochemical Analysis
4.4. Measurement of Physio-Biochemical Parameters
4.5. TUNEL Assays
4.6. Transmission Electron Microscopy
4.7. Darkness and ABA treatment
4.8. ABA Content Measurement
4.9. Real-Time Fluorescent Quantitative PCR (qRT-PCR) Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Material | Plant Height (cm) | Panicle Length (cm) | No. Panicle | 1000-Grain Weight (g) | Seed-Setting Rate (%) |
---|---|---|---|---|---|
ZJ100 | 101.66 ± 2.89a | 26.60 ± 0.53a | 14.67 ± 0.58b | 22.01 ± 0.10a | 85.99 ± 0.86a |
M15 | 97.50 ± 1.82ab | 24.00 ± 0.50bc | 11.00 ± 1.00de | 21.48 ± 0.12b | 76.33 ± 0.75c |
M50 | 97.83 ± 1.76ab | 23.73 ± 0.72bc | 10.33 ± 0.58e | 19.53 ± 0.09d | 74.88 ± 0.46c |
M89 | 95.17 ± 2.25b | 19.63 ± 1.23d | 13.33 ± 1.15bc | 18.80 ± 0.07e | 81.14 ± 2.08b |
M117 | 83.33 ± 4.72c | 22.33 ± 0.86c | 16.67 ± 1.15a | 20.84 ± 0.20c | 74.27 ± 1.63c |
M270 | 93.67 ± 4.62b | 24.73 ± 1.46b | 12.33 ± 1.15cd | 21.74 ± 0.32ab | 86.32 ± 2.41a |
Cross | F1 | No. F2 Individual | χ2 (3:1) | |
---|---|---|---|---|
Wild-Type | Mutant-Type | |||
psl15/ZJ100 | Normal | 292 | 88 | 0.69 |
psl50/ZJ100 | Normal | 495 | 170 | 0.11 |
psl89/IR64 | Normal | 457 | 137 | 1.19 |
psl117/ZJ100 | Normal | 501 | 154 | 0.77 |
psl270/80A90YR72 | Normal | 225 | 63 | 1.50 |
Cross (Female Parent × Male Parent) | Phenotype of F1 Plants | Allelism |
---|---|---|
psl15 × psl50 | Wild-type | Non-allelic |
psl89 × psl15 | Non-survival | Not determined |
psl117 × psl15 | Wild-type | Non-allelic |
psl270 × psl15 | Wild-type | Non-allelic |
psl50 × psl89 | Wild-type | Non-allelic |
psl117 × psl50 | Non-survival | Not determined |
psl270 × psl50 | Wild-type | Non-allelic |
psl89 × psl117 | Wild-type | Non-allelic |
psl89 × psl270 | Wild-type | Non-allelic |
psl117 × psl270 | Wild-type | Non-allelic |
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He, Y.; Li, L.; Zhang, Z.; Wu, J.-L. Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2018, 19, 140. https://doi.org/10.3390/ijms19010140
He Y, Li L, Zhang Z, Wu J-L. Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.). International Journal of Molecular Sciences. 2018; 19(1):140. https://doi.org/10.3390/ijms19010140
Chicago/Turabian StyleHe, Yan, Liangjian Li, Zhihong Zhang, and Jian-Li Wu. 2018. "Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)" International Journal of Molecular Sciences 19, no. 1: 140. https://doi.org/10.3390/ijms19010140