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Int. J. Mol. Sci. 2018, 19(1), 138; https://doi.org/10.3390/ijms19010138

Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein

1
School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China
2
Department of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China
*
Author to whom correspondence should be addressed.
Received: 23 November 2017 / Revised: 14 December 2017 / Accepted: 20 December 2017 / Published: 4 January 2018
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Abstract

The blue mussel (Mytilus edulis) reportedly contains many bioactive components of nutritional value. Water-, salt- and acid-soluble M. edulis protein fractions were obtained and the proteins were trypsinized. The resultant peptides were analyzed by ultra-performance liquid chromatography quadrupole time of flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). 387 unique peptides were identified that matched 81 precursor proteins. Molecular mass distributions of the proteins and peptides were analyzed by sodium dodecyl sulfate-polyacryl amide gel electrophoresis (SDS-PAGE). The differences between the three protein samples were studied by Venn diagram of peptide and protein compositions. Toxicity, allergic and antithrombotic activity of peptides was predicted using database website and molecular docking respectively. The antithrombotic activity of enzymatic hydrolysate from water-, salt- and acid-soluble M. edulis protein were 40.17%, 85.74%, 82.00% at 5 mg/mL, respectively. Active mechanism of antithrombotic peptide (ELEDSLDSER) was also research about amino acid binding sites and interaction, simultaneously. View Full-Text
Keywords: Mytilus edulis; protein; enzymatic hydrolysis; UPLC-Q-TOF-MS/MS; in silico analysis Mytilus edulis; protein; enzymatic hydrolysis; UPLC-Q-TOF-MS/MS; in silico analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Qiao, M.; Tu, M.; Wang, Z.; Mao, F.; Chen, H.; Qin, L.; Du, M. Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein. Int. J. Mol. Sci. 2018, 19, 138.

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