Differential Characterization of Temozolomide-Resistant Human Glioma Cells
Abstract
:1. Introduction
2. Results
2.1. MGMT and Cx43 Are Upregulated in the TMZ-Resistant U251 Cells
2.2. The TMZ-Resistant Subline and the Parental Cells Exhibited Differential Migratory Abilities and Protein Expression Profiles
2.3. The TMZ-Resistant Subline Showed Decreased Monocyte Adhesion Ability and the Differential Expression of Proliferation-Related Proteins
2.4. The TMZ-Resistant Subline Exhibited Lower Sensitivity to TNFα-Induction
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Monocyte-Binding Assay
4.4. Flow Cytometry Assay
4.5. Western Blot
4.6. RNA Extraction and Quantitative Real-Time PCR (q-PCR)
4.7. Wound-Healing Assay
4.8. Sulforhodamine B Assay (SRB)
4.9. PrestoBlue Proliferation Assay
4.10. Statistics
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lai, S.-W.; Huang, B.-R.; Liu, Y.-S.; Lin, H.-Y.; Chen, C.-C.; Tsai, C.-F.; Lu, D.-Y.; Lin, C. Differential Characterization of Temozolomide-Resistant Human Glioma Cells. Int. J. Mol. Sci. 2018, 19, 127. https://doi.org/10.3390/ijms19010127
Lai S-W, Huang B-R, Liu Y-S, Lin H-Y, Chen C-C, Tsai C-F, Lu D-Y, Lin C. Differential Characterization of Temozolomide-Resistant Human Glioma Cells. International Journal of Molecular Sciences. 2018; 19(1):127. https://doi.org/10.3390/ijms19010127
Chicago/Turabian StyleLai, Sheng-Wei, Bor-Ren Huang, Yu-Shu Liu, Hsiao-Yun Lin, Chun-Chuan Chen, Cheng-Fang Tsai, Dah-Yuu Lu, and Chingju Lin. 2018. "Differential Characterization of Temozolomide-Resistant Human Glioma Cells" International Journal of Molecular Sciences 19, no. 1: 127. https://doi.org/10.3390/ijms19010127