Differences in Sulfotyrosine Binding amongst CXCR1 and CXCR2 Chemokine Ligands
Abstract
:1. Introduction
2. Results
2.1. Sulfotyrosine Recognition Sites Correspond to Predicted Chemokine Hot Spots
2.2. NMR Titration Studies with Sulfotyrosine
2.3. Binding Affinity to Sulfotyrosine
3. Discussion
4. Materials and Methods
4.1. FTMap
4.2. Protein Expression and Purification
4.3. NMR Spectroscopy
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
CXCL | CXC ligand |
CXCR | CXC receptor |
CCL | CC ligand |
GPCR | G-protein Coupled Receptor |
sY | Sulfotyrosine |
NMR | Nuclear Magnetic Resonance |
HSQC | Heteronuclear Single Quantum Coherence |
CSP | Chemical Shift Perturbation |
ELR+ | Glutamate-leucine-arginine positive |
GAG | Glycosaminoglycan |
SPR | Surface Plasmon Resonance |
Appendix A
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Moussouras, N.A.; Getschman, A.E.; Lackner, E.R.; Veldkamp, C.T.; Dwinell, M.B.; Volkman, B.F. Differences in Sulfotyrosine Binding amongst CXCR1 and CXCR2 Chemokine Ligands. Int. J. Mol. Sci. 2017, 18, 1894. https://doi.org/10.3390/ijms18091894
Moussouras NA, Getschman AE, Lackner ER, Veldkamp CT, Dwinell MB, Volkman BF. Differences in Sulfotyrosine Binding amongst CXCR1 and CXCR2 Chemokine Ligands. International Journal of Molecular Sciences. 2017; 18(9):1894. https://doi.org/10.3390/ijms18091894
Chicago/Turabian StyleMoussouras, Natasha A., Anthony E. Getschman, Emily R. Lackner, Christopher T. Veldkamp, Michael B. Dwinell, and Brian F. Volkman. 2017. "Differences in Sulfotyrosine Binding amongst CXCR1 and CXCR2 Chemokine Ligands" International Journal of Molecular Sciences 18, no. 9: 1894. https://doi.org/10.3390/ijms18091894