Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis
Abstract
:1. Introduction
2. Results
2.1. Macroscopic and Histological Findings
2.2. Unique Expression Pattern of Exosomal miRNA in Reflux Esophagitis
2.3. The Specificity of Exosomal miR-29a-3p with Reflux Esophagitis
2.4. miRNA and mRNA Expression Analysis in Esophageal Tissue by Real-Time RT-PCR
2.5. The Relationship between miR-223-3p and mRNA in Esophageal Tissue
3. Discussion
4. Materials and Methods
4.1. Animals and Induction of Esophagitis, Gastric Ulcers and Colitis
4.2. Isolation of Serum Exosomes and RNA Extraction
4.3. Microarray Analysis
4.4. Real-Time qPCR for miRNA
4.5. Real-Time qPCR for mRNA
4.6. Statistical Analyses
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
GERD | Gastroesophageal reflux disease |
ERD | Erosive reflux disease |
NERD | Non-erosive reflux disease |
PPI | Proton pump inhibitor |
miRNA | MicroRNA |
DSS | Dextran sulfate sodium |
IL-1β | Interleukin-1β |
COX-2 | Cyclooxygenase-2 |
STAT3 | Transcription 3 |
ESCC | Esophageal squamous cell carcinoma |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase |
SE | Standard error |
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Uemura, R.; Murakami, Y.; Hashimoto, A.; Sawada, A.; Otani, K.; Taira, K.; Hosomi, S.; Nagami, Y.; Tanaka, F.; Kamata, N.; et al. Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis. Int. J. Mol. Sci. 2017, 18, 1611. https://doi.org/10.3390/ijms18081611
Uemura R, Murakami Y, Hashimoto A, Sawada A, Otani K, Taira K, Hosomi S, Nagami Y, Tanaka F, Kamata N, et al. Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis. International Journal of Molecular Sciences. 2017; 18(8):1611. https://doi.org/10.3390/ijms18081611
Chicago/Turabian StyleUemura, Risa, Yoshiki Murakami, Atsushi Hashimoto, Akinari Sawada, Koji Otani, Koichi Taira, Shuhei Hosomi, Yasuaki Nagami, Fumio Tanaka, Noriko Kamata, and et al. 2017. "Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis" International Journal of Molecular Sciences 18, no. 8: 1611. https://doi.org/10.3390/ijms18081611