Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis
AbstractGastroesophageal reflux disease (GERD) is a common upper gastrointestinal disease. However, the role of exosomal microRNAs (miRNAs) and esophageal miRNAs in GERD has not been studied. A rat model of acid reflux esophagitis was used to establish a novel diagnosis marker for GERD and examine dynamics of miRNA expression in GERD. Rats were sacrificed 3 (acute phase), 7 (sub-acute phase) and 21 days (chronic phase) after induction of esophagitis. Exosomes were extracted from serum, and the expression patterns of serum miRNAs were analyzed. Four upregulated miRNAs (miR-29a-3p, 128-3p, 223-3p and 3473) were identified by microarray analysis. The expression levels of exosomal miR-29a-3p were significantly higher in the chronic phase of reflux esophagitis compared with controls, and increased expression of miR-29a-3p was specific to chronic reflux esophagitis. Esophageal miR-223-3p expression was higher compared with controls, and gradually decreased from acute to chronic phase in esophagitis. In conclusion, exosomal miR-29a-3p and esophageal miR-223-3p might play roles in GERD. View Full-Text
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Uemura, R.; Murakami, Y.; Hashimoto, A.; Sawada, A.; Otani, K.; Taira, K.; Hosomi, S.; Nagami, Y.; Tanaka, F.; Kamata, N.; Yamagami, H.; Tanigawa, T.; Watanabe, T.; Taguchi, Y.-H.; Fujiwara, Y. Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis. Int. J. Mol. Sci. 2017, 18, 1611.
Uemura R, Murakami Y, Hashimoto A, Sawada A, Otani K, Taira K, Hosomi S, Nagami Y, Tanaka F, Kamata N, Yamagami H, Tanigawa T, Watanabe T, Taguchi Y-H, Fujiwara Y. Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis. International Journal of Molecular Sciences. 2017; 18(8):1611.Chicago/Turabian Style
Uemura, Risa; Murakami, Yoshiki; Hashimoto, Atsushi; Sawada, Akinari; Otani, Koji; Taira, Koichi; Hosomi, Shuhei; Nagami, Yasuaki; Tanaka, Fumio; Kamata, Noriko; Yamagami, Hirokazu; Tanigawa, Tetsuya; Watanabe, Toshio; Taguchi, Y-h; Fujiwara, Yasuhiro. 2017. "Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis." Int. J. Mol. Sci. 18, no. 8: 1611.
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