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A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.)

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(7), 1376;
Received: 11 May 2017 / Revised: 23 June 2017 / Accepted: 23 June 2017 / Published: 27 June 2017
(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
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PKA (protein lysine acetylation) is a critical post-translational modification that regulates various developmental processes, including seed development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early seed development. We report the first quantitative acetylproteomic study focused on rice early seed development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early seed development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early seed development. View Full-Text
Keywords: Rice (Oryza sativa L.); acetylome; early seed development; post-translational modification Rice (Oryza sativa L.); acetylome; early seed development; post-translational modification

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Wang, Y.; Hou, Y.; Qiu, J.; Li, Z.; Zhao, J.; Tong, X.; Zhang, J. A Quantitative Acetylomic Analysis of Early Seed Development in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2017, 18, 1376.

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