Effects of Pup Separation on Stress Response in Postpartum Female Rats
Abstract
:1. Introduction
2. Results
2.1. Circulating CORT Levels of Virgin and Lactating Rats with or without Pup Separation Following No-Stress or an Acute Stress
2.2. Circulating PRL Levels of Virgin and Lactating Rats with or without Pup Separation Following No-Stress or an Acute Stress
2.3. PRL-R mRNA Levels of Virgin and Lactating Rats with or without Pup Separation Following No-Stress or an Acute Stress
2.4. Correlation Among CORT, PRL, and PRL-R mRNA Levels of Virgin and Lactating Rats with or without Pup Separation Following No-Stress or an Acute Stress
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Restraint Stress and Sample Collection
4.3. Hormone Assays
4.4. Quantitative RT-PCR
4.5. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
icv | Cerebroventricle infusion |
ACTH | Adrenocorticotropic hormone |
CNS | Central nervous system |
CORT | Corticosterone |
CRH | Corticotropin releasing hormone |
HPA | Hypothalamic-pituitary-adrenal |
PRL | Prolactin |
PRL-R | Prolactin receptor |
PVN | Paraventricular nucleus |
qRT-PCR | Quantitative real-time PCR |
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Kalyani, M.; Callahan, P.; Janik, J.M.; Shi, H. Effects of Pup Separation on Stress Response in Postpartum Female Rats. Int. J. Mol. Sci. 2017, 18, 1370. https://doi.org/10.3390/ijms18071370
Kalyani M, Callahan P, Janik JM, Shi H. Effects of Pup Separation on Stress Response in Postpartum Female Rats. International Journal of Molecular Sciences. 2017; 18(7):1370. https://doi.org/10.3390/ijms18071370
Chicago/Turabian StyleKalyani, Manu, Phyllis Callahan, James M. Janik, and Haifei Shi. 2017. "Effects of Pup Separation on Stress Response in Postpartum Female Rats" International Journal of Molecular Sciences 18, no. 7: 1370. https://doi.org/10.3390/ijms18071370