Next Article in Journal
Melatonin Promotes the In Vitro Development of Microinjected Pronuclear Mouse Embryos via Its Anti-Oxidative and Anti-Apoptotic Effects
Next Article in Special Issue
Pathological Analysis of Ocular Lesions in a Murine Model of Sjögren’s Syndrome
Previous Article in Journal
Protective Effect of Fragaria ananassa Crude Extract on Cadmium-Induced Lipid Peroxidation, Antioxidant Enzymes Suppression, and Apoptosis in Rat Testes
Previous Article in Special Issue
A Comparison of the Effects of Benzalkonium Chloride on Ocular Surfaces between C57BL/6 and BALB/c Mice
Open AccessArticle

Goblet Cells Contribute to Ocular Surface Immune Tolerance—Implications for Dry Eye Disease

1
Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
2
Department of Ophthalmology, the Second Xiangya Hospital, Central South University, Changsha 410011, China
3
Department of Ophthalmology, Konyang University Hospital, College of Medicine, Konyang University, Daejeon 302-718, Korea
4
Hubrecht Institute, 3584 CT Utrecht, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Igor A. Butovich
Int. J. Mol. Sci. 2017, 18(5), 978; https://doi.org/10.3390/ijms18050978
Received: 8 April 2017 / Revised: 27 April 2017 / Accepted: 2 May 2017 / Published: 5 May 2017
(This article belongs to the Special Issue Dry Eye and Ocular Surface Disorders)
Conjunctival goblet cell (GC) loss in dry eye is associated with ocular surface inflammation. This study investigated if conjunctival GCs contribute to ocular surface immune tolerance. Antigens applied to the ocular surface, imaged by confocal microscopy, passed into the conjunctival stroma through goblet cell associated passages (GAPs) in wild type C57BL/6 (WT), while ovalbumin (OVA) was retained in the epithelium of SAM pointed domain containing ETS transcription factor (Spdef) knockout mice (Spdef/) that lack GCs and are a novel model of dry eye. Stimulated GC degranulation increased antigen binding to GC mucins. Induction of tolerance to topically applied OVA measured by cutaneous delayed type hypersensitivity (DTH) was observed in WT, but not Spdef/. OTII CD4+ T cells primed by dendritic cells (DCs) from the conjunctival draining lymph nodes of Spdef/ had greater IFN-γ production and lower Foxp3 positivity than those primed by WT DCs. These findings indicate that conjunctival GCs contribute to ocular surface immune tolerance by modulating antigen distribution and antigen specific immune response. GC loss may contribute to the abrogation of ocular surface immune tolerance that is observed in dry eye. View Full-Text
Keywords: mucins; antigen; goblet cell; dendritic cells; adaptive immunity; immune tolerance mucins; antigen; goblet cell; dendritic cells; adaptive immunity; immune tolerance
Show Figures

Figure 1

MDPI and ACS Style

Barbosa, F.L.; Xiao, Y.; Bian, F.; Coursey, T.G.; Ko, B.Y.; Clevers, H.; De Paiva, C.S.; Pflugfelder, S.C. Goblet Cells Contribute to Ocular Surface Immune Tolerance—Implications for Dry Eye Disease. Int. J. Mol. Sci. 2017, 18, 978.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop