Magnetically Bioprinted Human Myometrial 3D Cell Rings as A Model for Uterine Contractility
Nano3D Biosciences, Houston, TX 77030, USA
Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology, and Reproductive Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030, USA
Author to whom correspondence should be addressed.
Academic Editor: William Chi-Shing Cho
Int. J. Mol. Sci. 2017, 18(4), 683; https://doi.org/10.3390/ijms18040683
Received: 8 March 2017 / Revised: 14 March 2017 / Accepted: 16 March 2017 / Published: 23 March 2017
(This article belongs to the Collection Precision Medicine—From Bench to Bedside)
Deregulation in uterine contractility can cause common pathological disorders of the female reproductive system, including preterm labor, infertility, inappropriate implantation, and irregular menstrual cycle. A better understanding of human myometrium contractility is essential to designing and testing interventions for these important clinical problems. Robust studies on the physiology of human uterine contractions require in vitro models, utilizing a human source. Importantly, uterine contractility is a three-dimensionally (3D)-coordinated phenomenon and should be studied in a 3D environment. Here, we propose and assess for the first time a 3D in vitro model for the evaluation of human uterine contractility. Magnetic 3D bioprinting is applied to pattern human myometrium cells into rings, which are then monitored for contractility over time and as a function of various clinically relevant agents. Commercially available and patient-derived myometrium cells were magnetically bioprinted into rings in 384-well formats for throughput uterine contractility analysis. The bioprinted uterine rings from various cell origins and patients show different patterns of contractility and respond differently to clinically relevant uterine contractility inhibitors, indomethacin and nifedipine. We believe that the novel system will serve as a useful tool to evaluate the physiology of human parturition while enabling high-throughput testing of multiple agents and conditions.