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Pre-Exposure Gene Expression in Baboons with and without Pancytopenia after Radiation Exposure

Bundeswehr Institute of Radiobiology, 80937 Munich, Germany
Institut de Recherche Biomedicale des Armees, 91220 Bretigny-sur-Orge, France
Author to whom correspondence should be addressed.
Academic Editor: Terrence Piva
Int. J. Mol. Sci. 2017, 18(3), 541;
Received: 27 January 2017 / Revised: 17 February 2017 / Accepted: 24 February 2017 / Published: 2 March 2017
(This article belongs to the Collection Radiation Toxicity in Cells)
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Radiosensitivity differs in humans and likely among primates. The reasons are not well known. We examined pre-exposure gene expression in baboons (n = 17) who developed haematologic acute radiation syndrome (HARS) without pancytopenia or a more aggravated HARS with pancytopenia after irradiation. We evaluated gene expression in a two stage study design where stage I comprised a whole genome screen for messenger RNAs (mRNA) (microarray) and detection of 667 microRNAs (miRNA) (real-time quantitative polymerase chain reaction (qRT-PCR) platform). Twenty candidate mRNAs and nine miRNAs were selected for validation in stage II (qRT-PCR). None of the mRNA species could be confirmed during the validation step, but six of the nine selected candidate miRNA remained significantly different during validation. In particular, miR-425-5p (receiver operating characteristic = 0.98; p = 0.0003) showed nearly complete discrimination between HARS groups with and without pancytopenia. Target gene searches of miR-425-5p identified new potential mRNAs and associated biological processes linked with radiosensitivity. We found that one miRNA species examined in pre-exposure blood samples was associated with HARS characterized by pancytopenia and identified new target mRNAs that might reflect differences in radiosensitivity of irradiated normal tissue. View Full-Text
Keywords: radiosensitivity; pancytopenia; miRNA; gene expression; haematologic acute radiation syndrome (HARS) radiosensitivity; pancytopenia; miRNA; gene expression; haematologic acute radiation syndrome (HARS)

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Port, M.; Hérodin, F.; Valente, M.; Drouet, M.; Ullmann, R.; Majewski, M.; Abend, M. Pre-Exposure Gene Expression in Baboons with and without Pancytopenia after Radiation Exposure. Int. J. Mol. Sci. 2017, 18, 541.

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