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Int. J. Mol. Sci. 2017, 18(12), 2715;

Femtograms of Interferon-γ Suffice to Modulate the Behavior of Jurkat Cells: A New Light in Immunomodulation

Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, I-20157 Milan, Italy
Clinical Research Unit, GUNA S.p.a., Via Palmanova, 71, 20132 Milan, Italy
Service of Biostatistics Hematology Department Niguarda Ca’ Granda Hospital, 20162 Milan, Italy
Author to whom correspondence should be addressed.
Received: 8 November 2017 / Revised: 5 December 2017 / Accepted: 12 December 2017 / Published: 15 December 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Since interferon-γ (IFN-γ) tunes both innate and adaptive immune systems, it was expected to enter clinical practice as an immunomodulatory drug. However, the use of IFN-γ has been limited by its dose-dependent side effects. Low-dose medicine, which is emerging as a novel strategy to treat diseases, might circumvent this restriction. Several clinical studies have proved the efficacy of therapies with a low dose of cytokines subjected to kinetic activation, while no in vitro data are available. To fill this gap, we investigated whether low concentrations, in the femtogram range, of kinetically activated IFN-γ modulate the behavior of Jurkat cells, a widely used experimental model that has importantly contributed to the present knowledge about T cell signaling. In parallel, IFN-γ in the nanogram range was used and shown to activate Signal transducer and activator of transcription (STAT)-1 and then to induce suppressor of cytokine signaling-1 (SOCS-1), which inhibits downstream signaling. When added together, femtograms of IFN-γ interfere with the transduction cascade activated by nanograms of IFN-γ by prolonging the activation of STAT-1 through the downregulation of SOCS-1. We conclude that femtograms of IFN-γ exert an immunomodulatory action in Jurkat cells. View Full-Text
Keywords: interferon-γ; Jurkat cells; immunomodulation; STAT-1; SOCS-1 interferon-γ; Jurkat cells; immunomodulation; STAT-1; SOCS-1

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Castiglioni, S.; Miranda, V.; Cazzaniga, A.; Campanella, M.; Nichelatti, M.; Andena, M.; Maier, J.A.M. Femtograms of Interferon-γ Suffice to Modulate the Behavior of Jurkat Cells: A New Light in Immunomodulation. Int. J. Mol. Sci. 2017, 18, 2715.

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