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Int. J. Mol. Sci. 2017, 18(12), 2688;

The Consequences of Being in an Infectious Biofilm: Microenvironmental Conditions Governing Antibiotic Tolerance

Costerton Biofilm Centre, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark
Department of Clinical Microbiology, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark
Marine Biology Section, Department of Biology, University of Copenhagen, DK-3000 Elsinore, Denmark
Climate Change Cluster, University of Technology Sydney, Ultimo NSW 2007, Australia
Author to whom correspondence should be addressed.
Received: 17 October 2017 / Revised: 27 November 2017 / Accepted: 6 December 2017 / Published: 12 December 2017
(This article belongs to the Special Issue Biofilm Formation)
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The main driver behind biofilm research is the desire to understand the mechanisms governing the antibiotic tolerance of biofilm-growing bacteria found in chronic bacterial infections. Rather than genetic traits, several physical and chemical traits of the biofilm have been shown to be attributable to antibiotic tolerance. During infection, bacteria in biofilms exhibit slow growth and a low metabolic state due to O2 limitation imposed by intense O2 consumption of polymorphonuclear leukocytes or metabolically active bacteria in the biofilm periphery. Due to variable O2 availability throughout the infection, pathogen growth can involve aerobic, microaerobic and anaerobic metabolism. This has serious implications for the antibiotic treatment of infections (e.g., in chronic wounds or in the chronic lung infection of cystic fibrosis patients), as antibiotics are usually optimized for aerobic, fast-growing bacteria. This review summarizes knowledge about the links between the microenvironment of biofilms in chronic infections and their tolerance against antibiotics. View Full-Text
Keywords: biofilm; microenvironmental; antibiotic tolerance biofilm; microenvironmental; antibiotic tolerance

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sønderholm, M.; Bjarnsholt, T.; Alhede, M.; Kolpen, M.; Jensen, P.Ø.; Kühl, M.; Kragh, K.N. The Consequences of Being in an Infectious Biofilm: Microenvironmental Conditions Governing Antibiotic Tolerance. Int. J. Mol. Sci. 2017, 18, 2688.

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